SIFT-MS Analysis of Nose-Exhaled Breath; Mouth Contamination and the Influence of Exercise

0421269 - ÚFCH JH 2014 RIV US eng J - Journal Article
Smith, D. - Chippendale, T. W. E. - Dryahina, Kseniya - Španěl, Patrik
SIFT-MS Analysis of Nose-Exhaled Breath; Mouth Contamination and the Influence of Exercise.
Current Analytical Chemistry. Roč. 9, č. 2013 (2013), s. 565-575. ISSN 1573-4110. E-ISSN 1875-6727
Institutional support: RVO:61388955
Keywords : selected ion flow tube mass spectrometry * breath analysis * nose exhalation
Subject RIV: CF - Physical ; Theoretical Chemistry
Impact factor: 1.194, year: 2013

The value of selected ion flow tube mass spectrometry, SIFT-MS, for the sampling and analysis of trace me- tabolites in both mouth-exhaled and nose-exhaled breath is discussed, and the latter is shown to circumvent oral contami- nation. Results of experiments are described in which increased levels of ammonia and ethanol deliberately generated in the oral cavity are clearly registered in mouth-exhaled breath but not in nose-exhaled breath. The value of the sampling methods that are used for the analysis of trace metabolites in both mouth-exhaled and nose-exhaled breath with selected ion flow tube mass spectrometry, SIFT-MS, is discussed, and their importance as a means to provide concentrations of truly endogenous compounds by circumventing their oral contamination is demonstrated. Further to this, data are pre- sented on the direct analysis by SIFT-MS of several compounds in the nose-exhaled breath of eight healthy volunteers during exercise on a stationary bicycle. Of note are the data on breath isoprene, which is seen to increase during exercise above the respective resting levels for all eight volunteers by a factor of 2 or 3 times, this being in accordance with previ- ous observations. Taken together, the results of this study show the value and simplicity of direct analysis of nose-exhaled breath by SIFT-MS, which can provide immediate results to the clinician and sports physiologists with no pain or discom- fort to the volunteer or patient.
Permanent Link: http://hdl.handle.net/11104/0227703