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The use of new surface-modified poly(2-hydroxyethyl methacrylate) hydrogels in tissue engineering: Treatment of the surface with fibronectin subunits versus Ac-CGGASIKVAVS-OH, cysteine, and 2-mercaptoethanol modification
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SYSNO ASEP 0397918 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The use of new surface-modified poly(2-hydroxyethyl methacrylate) hydrogels in tissue engineering: Treatment of the surface with fibronectin subunits versus Ac-CGGASIKVAVS-OH, cysteine, and 2-mercaptoethanol modification Author(s) Kubinová, Šárka (UEM-P) RID, ORCID
Horák, Daniel (UMCH-V) RID, ORCID
Vaněček, Václav (UEM-P)
Plichta, Zdeněk (UMCH-V)
Proks, Vladimír (UMCH-V) RID, ORCID
Syková, Eva (UEM-P) RIDNumber of authors 6 Source Title Journal of Biomedical Materials Research. Part A. - : Wiley - ISSN 1549-3296
Roč. 102, č. 7 (2014), s. 2315-2323Number of pages 9 s. Language eng - English Country US - United States Keywords 2-hydroxyethyl methacrylate ; IKVAV (Ile-Lys-Val-Ala-Val) peptide ; fibronectin Subject RIV FH - Neurology Subject RIV - cooperation Institute of Macromolecular Chemistry - Macromolecular Chemistry R&D Projects GAP304/11/0731 GA ČR - Czech Science Foundation (CSF) GAP304/11/0653 GA ČR - Czech Science Foundation (CSF) GA13-00939S GA ČR - Czech Science Foundation (CSF) Institutional support UEM-P - RVO:68378041 ; UMCH-V - RVO:61389013 UT WOS 000337570400028 EID SCOPUS 84883140441 DOI 10.1002/jbm.a.34910 Annotation The objective of this study was to investigate the cell-adhesive properties of biomimetic ligands, such as laminin-derived Ac-CGGASIKVAVS-OH (SIKVAV) peptide and fibronectin subunits (Fn), as well as small molecules exemplified by 2-mercaptoethanol (ME) and cysteine (Cys), immobilized on a copolymer of 2-hydroxyethyl methacrylate (HEMA) with 2-aminoethyl methacrylate (AEMA) by a maleimide-thiol coupling reaction. The results show that the immobilization of SIKVAV and Fn-subunits onto superporous P(HEMA-AEMA) hydrogels via a GMBS coupling reaction improves cell adhesive properties. The high proliferative activity observed on Fn-modified hydrogels suggests that the immobilized Fn-subunits maintain their bioactivity and thus represent a promising tool for application in tissue engineering Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2015
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