The unique role of dietary L-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin

0392737 - BFÚ 2014 RIV US eng J - Journal Article
Pekarová, Michaela - Kubala, Lukáš - Papežíková, Ivana - Králová, Stanislava - Baldus, S. - Klinke, A. - Kuchta, R. - Kadlec, J. - Kuchtová, Z. - Kolářová, Hana - Lojek, Antonín
The unique role of dietary L-arginine in the acceleration of peritoneal macrophage sensitivity to bacterial endotoxin.
Immunologic Research. Roč. 56, č. 1 (2013), s. 73-84. ISSN 0257-277X. E-ISSN 1559-0755
R&D Projects: GA ČR(CZ) GA524/08/1753
Institutional research plan: CEZ:AV0Z50040702
Institutional support: RVO:68081707
Keywords : NITRIC-OXIDE-SYNTHASE * SUPEROXIDE ANION PRODUCTION * AMINO-ACID TRANSPORTERS
Subject RIV: BO - Biophysics
Impact factor: 3.525, year: 2013

It is known that cells and organisms can indirectly "sense" changes in l-arginine availability via changes in the activity of various metabolic pathways. However, the mechanism(s) by which genes can be directly regulated by l-arginine in mammalian cells have not yet been elucidated. We investigated the effect of l-arginine in the in vivo model of peritoneal inflammation in mice and in vitro in RAW 264.7 macrophages. A detailed analysis of basic physiological functions and selected intracellular signaling cascades revealed that l-arginine is crucial for the acceleration of macrophage activation by bacterial lipopolysaccharide. l-arginine increased the production of reactive oxygen species, nitric oxide, release of Ca2+, as well as inducible nitric oxide synthase expression. Interestingly, the effect of l-arginine on macrophage activation was dependent on the phosphorylation of mitogen-activated protein kinases and activity of phospholipase C.
Permanent Link: http://hdl.handle.net/11104/0221533