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Raman microspectroscopy of algal lipid bodies: beta-carotene quantification
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SYSNO ASEP 0383732 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Raman microspectroscopy of algal lipid bodies: beta-carotene quantification Author(s) Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Bernatová, Silvie (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Šerý, Mojmír (UPT-D) RID, SAI
Samek, Ota (UPT-D) RID, ORCID, SAI
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Nedbal, Ladislav (UEK-B)
Trtílek, M. (CZ)Number of authors 8 Source Title Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 24, č. 3 (2012), s. 541-546Number of pages 6 s. Language eng - English Country US - United States Keywords Raman microspectroscopy ; Microalgae ; Trachydiscus minutus ; Biotechnology ; Carotenoids Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects FR-TI1/433 GA MPO - Ministry of Industry and Trade (MPO) ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UPT-D - RVO:68081731 ; RVO:67179843 - RVO:67179843 UT WOS 000304187100029 DOI 10.1007/s10811-011-9754-4 Annotation Advanced optical instruments can serve for analysis and manipulation of individual living cells and their internal structures. We have used Raman microspectroscopic analysis for assessment of beta-carotene concentration in algal lipid bodies (LBs) in vivo. Some algae contain beta-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have devised a simple method to measure the concentration of beta-carotene in a mixture of algal storage lipids from the ratio of their Raman vibrations. This finding may allow fast acquisition of beta-carotene concentration valuable, e.g., for Raman microspectroscopy assisted cell sorting for selection of the overproducing strains. Furthermore, we demonstrate that beta-carotene concentration can be proportional to LB volume and light intensity during the cultivation. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on the spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2013
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