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Daxx inhibits stress-induced apoptosis in cardiac myocytes
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SYSNO ASEP 0325125 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Daxx inhibits stress-induced apoptosis in cardiac myocytes Title Daxx inhibuje apoptózu indukovanou stresem v kardiomyocytech Author(s) Zobalová, Renata (BTO-N) RID
Swettenham, E. (AU)
Chladová, Jaromíra (BTO-N) RID
Dong, L.F. (AU)
Neužil, Jiří (BTO-N) RIDSource Title Redox Report. - : Taylor & Francis - ISSN 1351-0002
Roč. 13, č. 6 (2008), s. 263-270Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords Daxx ; apoptosis ; oxidative stress Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA305/07/1008 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50520701 - BTO-N (2007-2013) Annotation The role of the death-associated protein Daxx in modulation of apoptosis induced in cardiac myocytes by oxidative stress was studied. Exposure of cardiomyocyte-like cells to oxidative stress or simulated hypoxia increased the level of accumulated ROS and apoptosis. Under conditions of sub-apoptotic stimulation of cardiac myocytes, there was no increase in the level of the Daxx protein, but it translocated from the nucleus to the cytoplasm. Daxx overexpression protected the cells from apoptosis, while they were sensitised to cell death following its down-regulation by siRNA. Lowering the level of the Daxx protein sensitised cardiac myocytes to spontaneous apoptosis, suggesting that the protein may also have a prosurvival role under physiological conditions. Finally, it was shown that DJ-1, a protein suggested previously to sequester Daxx in the nucleus under conditions of oxidative stress preventing thus its cytosolic translocation, was localised in the cytoplasm of cardiac myocytes Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2009
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