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Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis

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    SYSNO ASEP0191590
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JOstatní články
    TitleCalibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis
    Author(s) Veselý, Pavel (UMG-J)
    Mikš, A. (CZ)
    Novák, J. (CZ)
    Boyde, A. (GB)
    Source TitleScanning - ISSN 0161-0457
    Roč. 25, - (2003), s. 230-239
    Number of pages10 s.
    Languageeng - English
    CountryUS - United States
    Keywordsfast intracellular motion ; living cell ů video rate confocal laser scanning microscopy
    Subject RIVEA - Cell Biology
    R&D ProjectsGA304/99/0368 GA ČR - Czech Science Foundation (CSF)
    CEZAV0Z5052915 - UMG-J
    AnnotationVideo Rate Confocal Laser Scanning Microscope (VRCLSM) Odyssey (Noran, Middleton, WI, USA) at the highest spatial and temporal resolution of back scattered light (BSL) imaging enabled regular observation of fast intracellular motion (FIM) first revealed in living neoplastic cells. However, the absence of an objective evaluation has hampered further study of the mechanisms and biological significance of FIM. A search for a suitable method led to correlation analysis. It was calibrated on Brownian motion and a known type of motion such as cell marginal ruffling compared with FIM. Therefore, several crucial incidences of FIM could be analyzed. Apart from an argument against viewing FIM as a manifestation of simple Brownian motion, the correlation analysis of FIM in the adjacent peripheries of a rat fibroblast and a K4 rat sarcoma cell confirmed the notion of higher and uneven distribution of velocity of FIM in a tumor cell so far shown in color coded images only.
    WorkplaceInstitute of Molecular Genetics
    ContactNikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
    Year of Publishing2004

Number of the records: 1  

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