Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis
1.
SYSNO ASEP
0191590
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Calibration and quantification of fast intracellular motion (FIM) in living cells using correlation analysis
Author(s)
Veselý, Pavel (UMG-J) Mikš, A. (CZ) Novák, J. (CZ) Boyde, A. (GB)
Source Title
Scanning
- ISSN 0161-0457
Roč. 25, - (2003), s. 230-239
Number of pages
10 s.
Language
eng - English
Country
US - United States
Keywords
fast intracellular motion ; living cell ů video rate confocal laser scanning microscopy
Subject RIV
EA - Cell Biology
R&D Projects
GA304/99/0368 GA ČR - Czech Science Foundation (CSF)
CEZ
AV0Z5052915 - UMG-J
Annotation
Video Rate Confocal Laser Scanning Microscope (VRCLSM) Odyssey (Noran, Middleton, WI, USA) at the highest spatial and temporal resolution of back scattered light (BSL) imaging enabled regular observation of fast intracellular motion (FIM) first revealed in living neoplastic cells. However, the absence of an objective evaluation has hampered further study of the mechanisms and biological significance of FIM. A search for a suitable method led to correlation analysis. It was calibrated on Brownian motion and a known type of motion such as cell marginal ruffling compared with FIM. Therefore, several crucial incidences of FIM could be analyzed. Apart from an argument against viewing FIM as a manifestation of simple Brownian motion, the correlation analysis of FIM in the adjacent peripheries of a rat fibroblast and a K4 rat sarcoma cell confirmed the notion of higher and uneven distribution of velocity of FIM in a tumor cell so far shown in color coded images only.
