The DNA binding of insect Fork head factors is strongly influenced by the negative cooperation of neighbouring bases

0136064 - ENTU-I 20033089 RIV GB eng J - Journal Article
Takiya, S. - Gaži, Michal - Mach, Václav
The DNA binding of insect Fork head factors is strongly influenced by the negative cooperation of neighbouring bases.
Insect Biochemistry and Molecular Biology. Roč. 33, - (2003), s. 1145-1154. ISSN 0965-1748. E-ISSN 1879-0240
R&D Projects: GA AV ČR KSK5052113; GA ČR GA301/96/0153; GA MŠMT ME 176
Institutional research plan: CEZ:AV0Z5007907
Keywords : Drosophila * bombys * Galleria
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.358, year: 2003

The Drosophila melanogaster Fork head and Bombyx mori SGF1/Fork head proteins are key regulators of tissue specific gene expression in the modified larval labial glands. Here we use the competitive electrophoretic mobility shift assay to create a detailed Fork head binding matrix and we investigate some unusual features of the Fork head interaction with DNA. We found that the Fork head-DNA interaction is context dependent-the binding specificity of the protein is partly determined by specific combinations of neighbouring bases. Although the total number of the sub-optimal dinucleotide steps is not high, the negative cooperation of neighbouring bases significantly contributes to the overall binding site specificity. Our results allow efficient recognition of insect Fork head binding sites and we show that the putative Fork head cognate elements preferentially accumulate in the near upstream region of genes abundantly expressed in the labial gland. (C) 2003 Elsevier Ltd. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0033825