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Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy
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SYSNO ASEP 0043199 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Probing diffusion laws within cellular membranes by Z-scan fluorescence correlation spectroscopy Title Výzkum difúzních zákonitostí buněčných membrán pomocí fluorescenční korelační spektroskopie Author(s) Humpolíčková, Jana (UFCH-W) RID
Gielen, E. (BE)
Benda, Aleš (UFCH-W) RID, ORCID
Fagulová, Veronika (UFCH-W)
Vercammen, J. (BE)
van de Ven, M. (BE)
Hof, Martin (UFCH-W) RID, ORCID
Ameloot, M. (BE)
Engelborghs, Y. (BE)Source Title Biophysical Journal. - : Cell Press - ISSN 0006-3495
Roč. 91, č. 3 (2006), L23-L25Number of pages 3 s. Language eng - English Country US - United States Keywords model membranes ; rafts ; organization Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GA203/05/2308 GA ČR - Czech Science Foundation (CSF) LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40400503 - UFCH-W (2005-2011) Annotation The plasma membrane of various mammalian cell types is heterogeneous in structure and may contain microdomains, which can impose constraints on the lateral diffusion of its constituents. Fluorescence correlation spectroscopy (FCS) can be used to investigate the dynamic properties of the plasma membrane of living cells. Very recently, Wawrezinieck et al. (Wawrezinieck, L., H. Rigneault, D. Marguet, and P. F. Lenne. 2005. Biophys. J. 89:4029-4042) described a method to probe the nature of the lateral microheterogeneities of the membrane by varying the beam size in the FCS instrument. The dependence of the width of the autocorrelation function at half-maximum, i.e., the diffusion time, on the transverse area of the confocal volume gives information on the nature of the imposed confinement. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2007
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