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Molecular and Biological Analysis of Potato virus M (PVM) Isolates from the Czech Republic
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SYSNO ASEP 0451389 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular and Biological Analysis of Potato virus M (PVM) Isolates from the Czech Republic Author(s) Plchová, Helena (UEB-Q) RID, ORCID
Vaculík, Petr (UEB-Q) RID
Čeřovská, Noemi (UEB-Q) RID, ORCID
Moravec, Tomáš (UEB-Q) RID, ORCID
Dědič, P. (CZ)Source Title Journal of Phytopathology - Phytopathologische Zeitschrift. - : Wiley - ISSN 0931-1785
Roč. 163, 11-12 (2015), s. 1031-1035Number of pages 5 s. Language eng - English Country DE - Germany Keywords Czech Republic ; phylogeny ; Potato virus M Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M06030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEB-Q - RVO:61389030 UT WOS 000363730100020 DOI 10.1111/jph.12334 Annotation The sequences of the 3-terminal region of four Czech Potato virus M isolates VIRUBRA 4/007, VIRUBRA 4/009, VIRUBRA 4/016 and VIRUBRA 4/035 were determined and compared with sequences of PVM isolates available in GenBank. Among the Czech isolates, VIRUBRA 4/007 and 4/016 as well as VIRUBRA 4/016 and 4/035 showed the highest nucleotide identity (93%). Isolates VIRUBRA 4/007, 4/016 and 4/035 were most similar to the PV0273 isolate from Germany and to the wild isolate from Russia. Interestingly, isolate VIRUBRA 4/009 significantly differed from the other three Czech isolates and was the only European isolate that showed the highest nucleotide identity with American isolates. Moreover, the PVM isolates from the Czech Republic and Germany differed in their host range. Phylogenetic analysis based on ORF5 coding for coat protein showed that the Czech isolates could be classified in two of the three groupings of the phylogenetic tree obtained. This is the first report on molecular and biological analysis of the genome sequences of PVM isolates from the Czech Republic. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2016
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