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Quantitative vessel mapping on increment cores: a critical comparison of image acquisition methods
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SYSNO ASEP 0617877 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Quantitative vessel mapping on increment cores: a critical comparison of image acquisition methods Author(s) Peters, R. L. (BE)
Klesse, S. (CH)
Van den Bulcke, J. (BE)
Jourdain, L. M. Y. (CH)
von Arx, G. (CH)
Anadon-Rosell, A. (DE)
Krejza, Jan (UEK-B) RID, ORCID, SAI
Kahmen, A. (CH)
Fonti, M. (CH)
Prendin, A. L. (IT)
Babst, F. (CH)
De Mil, T. (BE)Number of authors 12 Article number 1502237 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 16, FEB (2025)Number of pages 15 s. Language eng - English Country CH - Switzerland Keywords wood anatomy ; earlywood vessels ; co2 enrichment ; ring anatomy ; responses ; drought ; climate ; tool ; densitometry ; chronologies ; broad-leaved species ; angiosperms ; quantitative wood anatomy ; x-ray CT scanning ; xylem porosity ; uncertainty analysis ; inter-and intra-annual variability ; radial profile Subject RIV EF - Botanics OECD category Environmental sciences (social aspects to be 5.7) R&D Projects LM2023048 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EH22_008/0004635 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEK-B - RVO:86652079 UT WOS 001431840400001 EID SCOPUS 85218880708 DOI https://doi.org/10.3389/fpls.2025.1502237 Annotation Introduction Quantitative wood anatomy is critical for establishing climate reconstruction proxies, understanding tree hydraulics, and quantifying carbon allocation. Its accuracy depends upon the image acquisition methods, which allows for the identification of the number and dimensions of vessels, fibres, and tracheids within a tree ring. Angiosperm wood is analysed with a variety of different image acquisition methods, including surface pictures, wood anatomical micro-sections, or X-ray computed micro-tomography. Despite known advantages and disadvantages, the quantitative impact of method selection on wood anatomical parameters is not well understood.Methods In this study, we present a systematic uncertainty analysis of the impact of the image acquisition method on commonly used anatomical parameters. We analysed four wood samples, representing a range of wood porosity, using surface pictures, micro-CT scans, and wood anatomical micro-sections. Inter-annual patterns were analysed and compared between methods from the five most frequently used parameters, namely mean lumen area (MLA), vessel density (VD), number of vessels (VN), mean hydraulic diameter (D h), and relative conductive area (RCA). A novel sectorial approach was applied on the wood samples to obtain intra-annual profiles of the lumen area (A l), specific theoretical hydraulic conductivity (K s), and wood density (rho).Results Our quantitative vessel mapping revealed that values obtained for hydraulic wood anatomical parameters are comparable across different methods, supporting the use of easily applicable surface picture methods for ring-porous and specific diffuse-porous tree species. While intra-annual variability is well captured by the different methods across species, wood density (rho) is overestimated due to the lack of fibre lumen area detection.Discussion Our study highlights the potential and limitations of different image acquisition methods for extracting wood anatomical parameters. Moreover, we present a standardized workflow for assessing radial tree ring profiles. These findings encourage the compilation of all studies using wood anatomical parameters and further research to refine these methods, ultimately enhancing the accuracy, replication, and spatial representation of wood anatomical studies. Workplace Global Change Research Institute Contact Nikola Šviková, svikova.n@czechglobe.cz, Tel.: 511 192 268 Year of Publishing 2026 Electronic address https://www.frontiersin.org/journals/plant-science/articles/10.3389/fpls.2025.1502237/full
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