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Fibrillar extracellular matrix produced by pericyte-like cells facilitates glioma cell dissemination
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SYSNO ASEP 0601846 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fibrillar extracellular matrix produced by pericyte-like cells facilitates glioma cell dissemination Author(s) Výmola, P. (CZ)
Garcia-Borja, E. (CZ)
Červenka, Jakub (UZFG-Y) ORCID
Balážiová, E. (CZ)
Vymolová, B. (CZ)
Vepřková, J. (CZ)
Vodička, Petr (UZFG-Y) ORCID
Skalníková, Helena (UZFG-Y) RID, ORCID
Tomáš, R. (CZ)
Netuka, D. (CZ)
Bušek, P. (CZ)
Šedo, A. (CZ)Article number 13265 Source Title Brain Pathology - ISSN 1015-6305
Roč. 34, č. 6 (2024)Number of pages 17 s. Publication form Online - E Language eng - English Country GB - United Kingdom Keywords collagen type I ; extracellular matrix proteins ; fibronectin ; glioblastoma ; pericytes ; proteomics OECD category Biochemistry and molecular biology R&D Projects LX22NPO5102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2023053 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000785 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UZFG-Y - RVO:67985904 UT WOS 001214406200001 EID SCOPUS 85192168720 DOI https://doi.org/10.1111/bpa.13265 Annotation Gliomagenesis induces profound changes in the composition of the extracellular matrix (ECM) of the brain. In this study, we identified a cellular population responsible for the increased deposition of collagen I and fibronectin in glioblastoma. Elevated levels of the fibrillar proteins collagen I and fibronectin were associated with the expression of fibroblast activation protein (FAP), which is predominantly found in pericyte-like cells in glioblastoma. FAP+ pericyte-like cells were present in regions rich in collagen I and fibronectin in biopsy material and produced substantially more collagen I and fibronectin in vitro compared to other cell types found in the GBM microenvironment. Using mass spectrometry, we demonstrated that 3D matrices produced by FAP+ pericyte-like cells are rich in collagen I and fibronectin and contain several basement membrane proteins. This expression pattern differed markedly from glioma cells. Finally, we have shown that ECM produced by FAP+ pericyte-like cells enhances the migration of glioma cells including glioma stem-like cells, promotes their adhesion, and activates focal adhesion kinase (FAK) signaling. Taken together, our findings establish FAP+ pericyte-like cells as crucial producers of a complex ECM rich in collagen I and fibronectin, facilitating the dissemination of glioma cells through FAK activation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2025 Electronic address https://onlinelibrary.wiley.com/doi/10.1111/bpa.13265
Number of the records: 1