Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing

0584926 - ÚMCH 2025 RIV CH eng J - Journal Article
Bandzerewicz, A. - Howis, J. - Wierzchowski, K. - Šlouf, Miroslav - Hodan, Jiří - Denis, P. - Golofit, T. - Pilarek, M. - Gadomska-Gajadhur, A.
Exploring the application of poly(1,2-ethanediol citrate)/polylactide nonwovens in cell culturing.
Frontiers in Bioengineering and Biotechnology. Roč. 12, 15 March (2024), č. článku 1332290. ISSN 2296-4185. E-ISSN 2296-4185
Institutional support: RVO:61389013
Keywords : citric acid * citrate-based polyesters * biomaterials
OECD category: Polymer science
Impact factor: 5.7, year: 2022
Method of publishing: Open access
https://www.frontiersin.org/articles/10.3389/fbioe.2024.1332290/full

Biomaterials containing citric acid as a building unit show potential for use as blood vessel and skin tissue substitutes. The success in commercializing implants containing a polymer matrix of poly(1,8-octanediol citrate) provides a rationale for exploring polycitrates based on other diols. Changing the aliphatic chain length of the diol allows functional design strategies to control the implant’s mechanical properties, degradation profile and surface energy. In the present work, poly(1,2-ethanediol citrate) was synthesized and used as an additive to polylactide in the electrospinning process. It was established that the content of polycitrate greatly influences the nonwovens’ properties: an equal mass ratio of polymers resulted in the best morphology. The obtained nonwovens were characterized by surface hydrophilicity, tensile strength, and thermal properties. L929 cell cultures were carried out on their surface. The materials were found to be non-cytotoxic and the degree of porosity was suitable for cell colonization. On the basis of the most important parameters for assessing the condition of cultured cells (cell density and viability, cell metabolic activity and lactate dehydrogenase activity), the potential of PLLA + PECit nonwovens for application in tissue engineering was established.
Permanent Link: https://hdl.handle.net/11104/0352706