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Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin
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SYSNO ASEP 0584554 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Preparative agarose gel electrophoresis for reducing matrix interferences of organoid cell medium prior to LC-MS analysis of insulin Author(s) Hrušková, H. (CZ)
Olsen, C. (NO)
Řemínek, Roman (UIACH-O) ORCID
Wang, C. (NO)
Aizenshtadt, A. (NO)
Krauss, S. (NO)
Scholz, H. (NO)
Roberg-Larsen, H. (NO)
Foret, František (UIACH-O) RID, ORCID
Wilson, S.R. (NO)Number of authors 10 Article number 464669 Source Title Journal of Chromatography A. - : Elsevier - ISSN 0021-9673
Roč. 1717, FEB (2024)Number of pages 7 s. Publication form Print - P Language eng - English Country NL - Netherlands Keywords proteins ; liquid chromatography-mass spectrometry ; preparative gel electrophoresis Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry Method of publishing Open access Institutional support UIACH-O - RVO:68081715 UT WOS 001173156700001 EID SCOPUS 85185009025 DOI 10.1016/j.chroma.2024.464669 Annotation Organoids are 3D cell cultures with microanatomies mimicking aspects of real organs, useful for e.g. animal free studies of development, disease, and drug discovery. The cell medium of organoid models of Langerhans islets, regulating blood glucose levels by insulin secretion, can be analyzed by liquid chromatography-mass spectrometry (LCMS). However, organoid medium complexity is a major challenge, as matrix interferences can reduce sensitivity and selectivity, even with optimized LCMS conditions. By applying preparative agarose gel electrophoresis-electrodialysis (PGE-ED), we were able to decrease the cell medium background signal, allowing for reduced interferences affecting LCMS analysis of human insulin. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2025 Electronic address https://www.sciencedirect.com/science/article/pii/S0021967324000426?via%3Dihub
Number of the records: 1