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Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus
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SYSNO ASEP 0584200 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Genomics and biochemical analyses reveal a metabolon key to β-L-ODAP biosynthesis in Lathyrus sativus Author(s) Edwards, A. (GB)
Njaci, I. (GB)
Sarkar, A. (GB)
Jiang, Z. (GB)
Kaithakottil, G. G. (GB)
Moore, C. (GB)
Cheema, J. (GB)
Stevenson, C. E. M. (GB)
Rejzek, M. (GB)
Novák, Petr (BC-A) RID, ORCID
Vigouroux, M. (GB)
Vickers, M. (GB)
Wouters, R. H. M. (GB)
Paajanen, P. (GB)
Steuernagel, B. (GB)
Moore, J. D. (GB)
Higgins, J. (GB)
Swarbreck, D. (DE)
Martens, S. (IT)
Kim, C. Y. (US)
Weng, J. (US)
Mundree, S. (AU)
Kilian, B. (DE)
Kumar, S. (MA)
Loose, M. (GB)
Yant, L. (GB)
Macas, Jiří (BC-A) RID, ORCID, SAI
Wang, T. L. (GB)
Martin, C. (GB)
Emmrich, P. M. F. (GB)Number of authors 30 Article number 5199 Source Title Nature Communications. - : Nature Publishing Group
Roč. 14, č. 1 (2023)Number of pages 15 s. Language eng - English Country US - United States Keywords Grass pea (Lathyrus sativus L.) ; L. sativus genome ; biosynthetic pathway ; neurotoxin Subject RIV EB - Genetics ; Molecular Biology OECD category Genetics and heredity (medical genetics to be 3) R&D Projects LM2018131 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support BC-A - RVO:60077344 UT WOS 001053826400011 EID SCOPUS 85148254990 DOI 10.1038/s41467-023-40984-6 Annotation Grass pea (Lathyrus sativus L.) is a rich source of protein cultivated as an insurance crop in Ethiopia, Eritrea, India, Bangladesh, and Nepal. Its resilience to both drought and flooding makes it a promising crop for ensuring food security in a changing climate. The lack of genetic resources and the crop’s association with the disease neurolathyrism have limited the cultivation of grass pea. Here, we present an annotated, long read-based assembly of the 6.5 Gbp L. sativus genome. Using this genome sequence, we have elucidated the biosynthetic pathway leading to the formation of the, β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP). The final reaction of the pathway depends on an interaction between L. sativus acyl-activating enzyme 3 (LsAAE3) and a BAHD-acyltransferase (LsBOS) that form a metabolon activated by CoA to produce β-L-ODAP. This provides valuable insight into the best approaches for developing varieties which produce substantially less toxin. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2024 Electronic address https://www.nature.com/articles/s41467-023-36503-2
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