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The disruption of circadian rhythmicity of gene expression in the hippocampus and associated structures in Gria2R/R mice, a comparison with C57BL/6J and Adar2−/− mice strains

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    SYSNO ASEP0583680
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleThe disruption of circadian rhythmicity of gene expression in the hippocampus and associated structures in Gria2R/R mice, a comparison with C57BL/6J and Adar2−/− mice strains
    Author(s) Lebedeva, M. (CZ)
    Kubištová, A. (CZ)
    Spišská, V. (CZ)
    Filipovská, E. (CZ)
    Pačesová, D. (CZ)
    Svobodová, I. (CZ)
    Kuchtiak, Viktor (FGU-C) ORCID
    Balík, Aleš (FGU-C) RID, ORCID
    Bendová, Z. (CZ)
    Article number148739
    Source TitleBrain Research. - : Elsevier - ISSN 0006-8993
    Roč. 1826, 1 March (2024)
    Number of pages5 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordscircadian rhythms ; Adar2 ; Gria2 ; mice ; hippocampus ; suprachiasmatic nucleus
    OECD categoryNeurosciences (including psychophysiology
    R&D ProjectsEF16_025/0007444 GA MZd - Ministry of Health (MZ)
    Institutional supportFGU-C - RVO:67985823
    UT WOS001165646600001
    EID SCOPUS85181063268
    DOI10.1016/j.brainres.2023.148739
    AnnotationAdar2-/- mice are a widely used model for studying the physiological consequences of reduced RNA editing. These mice are viable only when the Q/R editing site of the Gria2 subunit of the AMPA receptor is constitutively mutated to the codon for arginine, and Gria2R/R mice often serve as the sole control for Adar2-/- mice. Our study aimed to investigate whether ADAR2 inactivity and the Gria2R/R phenotype affect the rhythmicity of the circadian clock gene pattern and the expression of Gria1 and Gria2 subunits in the suprachiasmatic nucleus (SCN), hippocampus, parietal cortex and liver. Our data show that Gria2R/R mice completely lost circadian rhythmicity in the hippocampus compared to Adar2-/- mice. Compared to C57BL/6J mice, the expression profiles in the hippocampus and parietal cortex of Gria2R/R mice differ to the same extent as in Adar2-/-. No alterations were detected in the circadian profiles in the livers. These data suggest that the natural gradual postnatal increase in the editing of the Q/R site of the Gria2 subunit may be important for the development of circadian clockwork in some brain structures, and the use of Gria2R/R mice as the only control to Adar2-/- mice in the experiments dependent on the hippocampus and parietal cortex should therefore be considered.
    WorkplaceInstitute of Physiology
    ContactLucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
    Year of Publishing2025
    Electronic addresshttps://doi.org/10.1016/j.brainres.2023.148739
Number of the records: 1  

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