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Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
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SYSNO ASEP 0579986 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency. Author(s) Pourali, Parastoo (MBU-M)
Dzmitruk, Volha (BTO-N)
Benada, Oldřich (MBU-M) ORCID, RID
Svoboda, Milan (UIACH-O) RID, ORCID
Benson, Veronika (MBU-M) RID, ORCIDSource Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 13, September 6 (2023), s. 14669Number of pages 14 s. Language eng - English Country US - United States Keywords gold nanoparticles ; capping agent ; biosynthesis ; nucleic acid transfection ; drug carriers OECD category Microbiology R&D Projects EH22_010/0002357 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LM2018127 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CIISB II - 90127 - Masarykova univerzita Method of publishing Open access Institutional support MBU-M - RVO:61388971 ; BTO-N - RVO:86652036 ; UIACH-O - RVO:68081715 UT WOS 001138051400001 EID SCOPUS 85170103294 DOI 10.1038/s41598-023-41567-7 Annotation In this study, gold nanoparticles produced by eukaryotic cell waste (AuNP), were analyzed as a transfection tool. AuNP were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS) were used before and after conjugation with different nucleic acid (NA) types. Graphite furnace atomic absorption spectroscopy (GF-AAS) was used to determine the AuNP concentration. Conjugation was detected by electrophoresis. Confocal microscopy and quantitative real-time PCR (qPCR) were used to assess transfection. TEM, SEM, and EDS showed 25nm AuNP with round shape. The amount of AuNP was 3.75±0.2g/L and FTIR proved conjugation of all NA types to AuNP. All the samples had a negative charge of36 to46mV. Confocal microscopy confirmed internalization of the ssRNA-AuNP into eukaryotic cells and qPCR confirmed release and activity of carried RNA. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2024 Electronic address https://www.nature.com/articles/s41598-023-41567-7
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