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Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.

  1. 1.
    SYSNO ASEP0579986
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleConjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency.
    Author(s) Pourali, Parastoo (MBU-M)
    Dzmitruk, Volha (BTO-N)
    Benada, Oldřich (MBU-M) ORCID, RID
    Svoboda, Milan (UIACH-O) RID, ORCID
    Benson, Veronika (MBU-M) RID, ORCID
    Source TitleScientific Reports. - : Nature Publishing Group - ISSN 2045-2322
    Roč. 13, September 6 (2023), s. 14669
    Number of pages14 s.
    Languageeng - English
    CountryUS - United States
    Keywordsgold nanoparticles ; capping agent ; biosynthesis ; nucleic acid transfection ; drug carriers
    OECD categoryMicrobiology
    R&D ProjectsEH22_010/0002357 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    LM2018127 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Research InfrastructureCIISB II - 90127 - Masarykova univerzita
    Method of publishingOpen access
    Institutional supportMBU-M - RVO:61388971 ; BTO-N - RVO:86652036 ; UIACH-O - RVO:68081715
    UT WOS001138051400001
    EID SCOPUS85170103294
    DOI10.1038/s41598-023-41567-7
    AnnotationIn this study, gold nanoparticles produced by eukaryotic cell waste (AuNP), were analyzed as a transfection tool. AuNP were produced by Fusarium oxysporum and analyzed by spectrophotometry, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy dispersive X-ray spectroscopy (EDS). Fourier transform infrared spectroscopy (FTIR) and dynamic light scattering (DLS) were used before and after conjugation with different nucleic acid (NA) types. Graphite furnace atomic absorption spectroscopy (GF-AAS) was used to determine the AuNP concentration. Conjugation was detected by electrophoresis. Confocal microscopy and quantitative real-time PCR (qPCR) were used to assess transfection. TEM, SEM, and EDS showed 25nm AuNP with round shape. The amount of AuNP was 3.75±0.2g/L and FTIR proved conjugation of all NA types to AuNP. All the samples had a negative charge of36 to46mV. Confocal microscopy confirmed internalization of the ssRNA-AuNP into eukaryotic cells and qPCR confirmed release and activity of carried RNA.
    WorkplaceInstitute of Microbiology
    ContactEliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231
    Year of Publishing2024
    Electronic addresshttps://www.nature.com/articles/s41598-023-41567-7
Number of the records: 1  

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