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Micro/nano-patterns for enhancing differentiation of human neural stem cells and fabrication of nerve conduits via soft lithography and 3D printing
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SYSNO ASEP 0577110 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Micro/nano-patterns for enhancing differentiation of human neural stem cells and fabrication of nerve conduits via soft lithography and 3D printing Author(s) Litowczenko, J. (PL)
Wychowaniec, J. K. (PL)
Zaleski, K. (PL)
Marczak, L. (PL)
Edwards-Gayle, C. J. C. (GB)
Tadyszak, Krzysztof (UMCH-V)
Maciejewska, B. M. (PL)Article number 213653 Source Title Biomaterials Advances. - : Elsevier
Roč. 154, November (2023)Number of pages 21 s. Language eng - English Country NL - Netherlands Keywords groove patterns ; neurite guidance cues ; 3D printing Subject RIV CD - Macromolecular Chemistry OECD category Polymer science Method of publishing Limited access Institutional support UMCH-V - RVO:61389013 UT WOS 001165425100001 EID SCOPUS 85174354020 DOI 10.1016/j.bioadv.2023.213653 Annotation Topographical cues on materials can manipulate cellular fate, particularly for neural cells that respond well to such cues. Utilizing biomaterial surfaces with topographical features can effectively influence neuronal differentiation and promote neurite outgrowth. This is crucial for improving the regeneration of damaged neural tissue after injury. Here, we utilized groove patterns to create neural conduits that promote neural differentiation and axonal growth. We investigated the differentiation of human neural stem cells (NSCs) on silicon dioxide groove patterns with varying height-to-width/spacing ratios. We hypothesize that NSCs can sense the microgrooves with nanoscale depth on different aspect ratio substrates and exhibit different morphologies and differentiation fate. A comprehensive approach was employed, analyzing cell morphology, neurite length, and cell-specific markers. These aspects provided insights into the behavior of the investigated NSCs and their response to the topographical cues. Three groove-pattern models were designed with varying height-to-width/spacing ratios of 80, 42, and 30 for groove pattern widths of 1 μm, 5 μm, and 10 μm and nanoheights of 80 nm, 210 nm, and 280 nm. Smaller groove patterns led to longer neurites and more effective differentiation towards neurons, whereas larger patterns promoted multidimensional differentiation towards both neurons and glia. We transferred these cues onto patterned polycaprolactone (PCL) and PCL-graphene oxide (PCL-GO) composite ‘stamps’ using simple soft lithography and reproducible extrusion 3D printing methods. The patterned scaffolds elicited a response from NSCs comparable to that of silicon dioxide groove patterns. The smallest pattern stimulated the highest neurite outgrowth, while the middle-sized grooves of PCL-GO induced effective synaptogenesis. We demonstrated the potential for such structures to be wrapped into tubes and used as grafts for peripheral nerve regeneration. Grooved PCL and PCL-GO conduits could be a promising alternative to nerve grafting. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2024 Electronic address https://www.sciencedirect.com/science/article/pii/S277295082300376X?via%3Dihub
Number of the records: 1