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Common Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley

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    SYSNO ASEP0576727
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleCommon Post-translational Modifications (PTMs) of Proteins: Analysis by Up-to-Date Analytical Techniques with an Emphasis on Barley
    Author(s) Bobálová, Janette (UIACH-O) RID, ORCID
    Strouhalová, Dana (UIACH-O) ORCID
    Bobál, P. (CZ)
    Number of authors3
    Source TitleJournal of Agricultural and Food Chemistry. - : American Chemical Society - ISSN 0021-8561
    Roč. 71, č. 41 (2023), s. 14825-14837
    Number of pages13 s.
    Publication formPrint - P
    Languageeng - English
    CountryUS - United States
    Keywordsbarley ; post-translational modification ; protein ; mass spectrometry
    Subject RIVCB - Analytical Chemistry, Separation
    OECD categoryAnalytical chemistry
    Method of publishingOpen access
    Institutional supportUIACH-O - RVO:68081715
    UT WOS001079010800001
    EID SCOPUS85174751774
    DOI https://doi.org/10.1021/acs.jafc.3c00886
    AnnotationPost-translational modifications (PTMs) of biomacromolecules can be useful for understanding the processes by which a relatively small number of individual genes in a particular genome can generate enormous biological complexity in different organisms. The proteomes of barley and the brewing process were investigated by different techniques. However, their diverse and complex PTMs remain understudied. As standard analytical approaches have limitations, innovative analytical approaches need to be developed and applied in PTM studies. To make further progress in this field, it is necessary to specify the sites of modification, as well as to characterize individual isoforms with increased selectivity and sensitivity. This review summarizes advances in the PTM analysis of barley proteins, particularly those involving mass spectrometric detection. Our focus is on monitoring phosphorylation, glycation, and glycosylation, which critically influence functional behavior in metabolism and regulation in organisms.
    WorkplaceInstitute of Analytical Chemistry
    ContactIveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234
    Year of Publishing2024
    Electronic addresshttps://pubs.acs.org/doi/10.1021/acs.jafc.3c00886
Number of the records: 1  

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