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Controlled natural selection of soil microbiome through plant-soil feedback confers resistance to a foliar pathogen
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SYSNO ASEP 0575729 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Controlled natural selection of soil microbiome through plant-soil feedback confers resistance to a foliar pathogen Author(s) Kalachova, Tetiana (UEB-Q) ORCID
Jindřichová, Barbora (UEB-Q) RID, ORCID
Burketová, Lenka (UEB-Q) RID, ORCID
Monard, C. (FR)
Blouin, M. (FR)
Jacquiod, S. (FR)
Ruelland, E. (FR)
Puga-Freitas, R. (FR)Number of authors 8 Source Title Plant and Soil - ISSN 0032-079X
Roč. 485, 1-2 (2023), s. 181-195Number of pages 15 s. Language eng - English Country NL - Netherlands Keywords Controlled natural selection ; Plant immunity ; Plant-microbiome interactions ; Pseudomonas syringae DC3000 ; Salicylic acid ; Soil suppressiveness OECD category Biochemical research methods R&D Projects EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000823385200001 EID SCOPUS 85134255256 DOI https://doi.org/10.1007/s11104-022-05597-w Annotation Background and aims: The rhizosphere microbiome has been shown to contribute to nutrient acquisition, protection against biotic and abiotic stresses and, ultimately, to changes in the development and physiology of plants. Here, using a controlled natural selection approach, we followed the microbial dynamics in the soil of Arabidopsis thaliana plants infected with the foliar pathogen Pseudomonas syringae DC3000 (Pst). Methods: Plants were iteratively cultivated on a pasteurised soil inoculated with the soil microbial community of the previous iteration isolated from the rhizosphere of plants infected with Pst (pst-line) or not (mock-line). Modification of soil microbial communities was assessed through an amplicon-based metagenomic analysis targeting bacterial and fungal diversity. Plant fitness and transcript abundance of stress hormone related genes were also analysed. Results: At the tenth and eleventh iterations respectively, we observed a reduction in disease severity of 81% and 85% in pst-lines as compared to mock-lines. These changes were associated with (i) an early induction of defence mechanisms mediated by salicylic acid, in pst-line as compared to mock-line, shown by the decrease in transcript abundance of salicylic acid related genes, whereas jasmonic acid, ethylene or abscisic acid related genes remained unchanged and (ii) a shift in soil bacterial, and not in fungal, composition. Conclusions: Our study suggests that these changes in soil bacterial composition are mediated by plant-soil feedback in response to Pst and resulted in an activation of SA-related immune response in the plant. This supports the concept of applying plant-soil feedbacks to enhance soil suppressiveness against foliar pathogens. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1007/s11104-022-05597-w
Number of the records: 1