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Biochemiluminescence sensing of protein oxidation by reactive oxygen species generated by pulsed electric field
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SYSNO ASEP 0574988 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Biochemiluminescence sensing of protein oxidation by reactive oxygen species generated by pulsed electric field Author(s) Vahalová, Petra (URE-Y)
Havelka, Daniel (URE-Y) RID
Vaněčková, Eva (UFCH-W) SAI, ORCID
Zakar, Tomáš (URE-Y)
Kolivoška, Viliam (UFCH-W) RID, ORCID
Cifra, Michal (URE-Y) RID, ORCID, SAINumber of authors 6 Article number 133676 Source Title Sensors and Actuators B - Chemical. - : Elsevier
Roč. 385, JUN 15 (2023)Number of pages 11 s. Publication form Online - E Language eng - English Country NL - Netherlands Keywords Chemiluminescence ; Pulsed electric field ; Proteins ; Albumin ; Sensing ; Monitoring Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) Subject RIV - cooperation J. Heyrovsky Institute of Physical Chemistry - Physical ; Theoretical Chemistry R&D Projects GX20-06873X GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support URE-Y - RVO:67985882 ; UFCH-W - RVO:61388955 UT WOS 000982222600001 EID SCOPUS 85150920169 DOI 10.1016/j.snb.2023.133676 Annotation Pulsed electric field (PEF) technology is an important emerging modality for both biomedicine and the food industry, yet the mechanisms of action of a PEF on a biomolecular level remain unclear. In order to gain a better understanding of this, a sensing platform was developed to enable sensitive detection of oxidative processes induced by the PEF. It was found that the application of PEF in phosphate buffer leads to the electrogeneration of reactive oxygen species (ROS) which in turn triggers intrinsic chemiluminescence. Furthermore, the presence of the bovine serum albumin (BSA) was found to increase the luminescence signal, which was attributed to biochemiluminescence due to the reactions of BSA with the electrogenerated ROS. A carbonyl determination assay was then used to confirm that the BSA molecules had indeed been oxidatively modified. We proposed a scheme describing the reactions leading from charge transfer at the anode/electrolyte interface to the actual photon emission, helping to elucidate the mechanisms of action of PEF on proteins. The results may to open up new avenues for the application of PEF technology. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2024 Electronic address DOI10.1016/j.snb.2023.133676
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