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The alga Bracteacoccus bullatus (Chlorophyceae) isolated from snow, as a source of oil comprising essential unsaturated fatty acids and carotenoids
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SYSNO ASEP 0574982 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The alga Bracteacoccus bullatus (Chlorophyceae) isolated from snow, as a source of oil comprising essential unsaturated fatty acids and carotenoids Author(s) Lukavský, Jaromír (BU-J) RID, ORCID
Kopecký, Jan (MBU-M)
Kubáč, David (MBU-M) RID, ORCID
Kvíderová, Jana (BU-J) RID, ORCID
Procházková, Lenka (BU-J) ORCID
Řezanka, Tomáš (MBU-M) ORCIDSource Title Journal of Applied Phycology. - : Springer - ISSN 0921-8971
Roč. 35, FEB 2023 (2023), s. 649-660Number of pages 12 s. Language eng - English Country DE - Germany Keywords Bracteacoccus bullatus ; Cryoseston ; Polyunsaturated fatty acids Subject RIV EA - Cell Biology OECD category Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology Subject RIV - cooperation Institute of Microbiology - Microbiology, Virology R&D Projects TE01020080 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) LTAIN19139 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) TN01000048 GA TA ČR - Technology Agency of the Czech Republic (TA ČR) Method of publishing Limited access Institutional support BU-J - RVO:67985939 ; MBU-M - RVO:61388971 UT WOS 000933270300002 EID SCOPUS 85148081516 DOI 10.1007/s10811-023-02916-1 Annotation A unicellular alga isolated from snow in the Sierra Nevada Mountains (Spain) was characterised using a polyphasic approach. Comparative analysis of ITS2 rDNA secondary structures identified the new culture (CCALA 1120 Cepák and Lukavský
Nova Hedwigia 94:163–173, 2012) as being conspecific with Bracteacoccus bullatus (Chlorophyceae). For the first time this study documented sexual reproduction as the pairing of gametes and also an-isogamy. Strain CCALA 1120 had a temperature optimum of growth about 21 °C and an irradiance optimum above 160 μmol photons m−2 s−1. It was cultivated in pilot-plant scale, using an open thin-layer photobioreactor in a greenhouse with only partial temperature control. After harvest, a high proportion of fatty acids was found (15.3% of dry mass) with linoleic (18:2ω-6) 18.3% and α-linolenic acids (18:3ω-3) 17.4%
being the most abundant. Monounsaturated fatty acids accounted for about 30% with oleic (18:1ω-9) and vaccenic acids (18:1 ω-7) as the most prominent. The ratio of PUFA ω-6/ω-3 was 1:1.16, i.e. near to the ideal ratio of 1:1, as recommended by the World Health Organization. Biomass production was 2.67 g m–2 day−1 of dry weight, i.e. 0.2 g L−1 day−1. At the end of growth phase, total carotenoids made up 10.1 mg L−1. These results indicate that B. bullatus is suitable for production of a vegetable oil at lower temperatures (12–18 °C) and comprising a high content of unsaturated fatty acids.
Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2024 Electronic address https://doi.org/10.1007/s10811-023-02916-1
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