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1,3-Selective hydrolysis of blackcurrant seed oil for the concentration of alpha- and gamma-linolenic acids
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SYSNO ASEP 0574067 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title 1,3-Selective hydrolysis of blackcurrant seed oil for the concentration of alpha- and gamma-linolenic acids Author(s) Sovová, Helena (UCHP-M) RID, ORCID, SAI
Topiař, Martin (UCHP-M) RID, ORCID, SAI
Pleskač, Ondřej (UCHP-M)Article number 105999 Source Title Journal of Supercritical Fluids. - : Elsevier - ISSN 0896-8446
Roč. 200, SEP (2023)Number of pages 8 s. Language eng - English Country NL - Netherlands Keywords blackcurrant seed oil ; linolenic acids ; hydrolysis OECD category Chemical engineering (plants, products) R&D Projects GA19-19245S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support UCHP-M - RVO:67985858 UT WOS 001013213500001 EID SCOPUS 85160698989 DOI 10.1016/j.supflu.2023.105999 Annotation Blackcurrant seed oil contains alpha-linolenic acid (a-Ln) and gamma-linolenic acid (g-Ln), essential fatty acids of different therapeutic effects. Lipid classes of various a-Ln/g-Ln ratios were previously produced by partial hydrolysis catalyzed by 1,3-specific lipases Lipozyme RM IM and Lipozyme TL IM, conducted in supercritical CO2 (scCO2) in a continuous packed-bed reactor. In this work, free fatty acids (FFA) were separated from glycerides
using preparative chromatography on silica gel with scCO2 as mobile phase in order to obtain two clean, natural products of different a-Ln/g-Ln ratios, and fatty acid profiles in hydrolysates obtained under different conditions were reviewed. Using Lipozyme RM IM and maintaining the FFA mass fraction in hydrolysate below that of partial glycerides, the a-Ln/g-Ln ratio increased from 1.3 in oil to 10.7 in FFA. Lipozyme RM IM-mediated hy-
drolysis showed steric hindrance of g-Ln, while silica support of Lipozyme TL IM promoted acyl migration.Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2024 Electronic address https://hdl.handle.net/11104/0344434
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