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Cryopreservation of Organoids
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SYSNO ASEP 0573693 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Cryopreservation of Organoids Author(s) Rogulska, Olena (FGU-C) ORCID, RID, SAI
Havelková, Jarmila (FGU-C)
Petrenko, Yuriy (FGU-C) ORCID, RID, SAISource Title Cryo-letters. - : CryoLetters LLP - ISSN 0143-2044
Roč. 44, č. 2 (2023), s. 65-75Number of pages 11 s. Language eng - English Country GB - United Kingdom Keywords organoids ; stem cells ; tumour ; cryopreservation ; vitrification ; recovery OECD category Biomaterials (as related to medical implants, devices, sensors) R&D Projects GA21-06065S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support FGU-C - RVO:67985823 UT WOS 001006587700001 EID SCOPUS 85152269856 DOI 10.54680/fr23210110112 Annotation Organoids represent indispensable opportunities for biomedicine, including drug discovery, cancer biology, regenerative and personalised medicine or tissue and organ transplantation. However, the lack of optimised preservation strategies limits the wide use of organoids in research or clinical fields. In this review, we present a short outline of the recent developments in organoid research and current cryopreservation strategies for organoid systems. While both vitrification and slow controlled freezing have been utilized for the cryopreservation of organoid structures or their precursor components, the controlled-rate slow freezing under protection of Me2 SO remains the most common approach. The application of appropriate pre- or post-treatment strategies, like the addition of Rho-kinase or myosin inhibitors into cell culture or cryopreservation medium, can increase the recovery of complex organoid constructs post-thaw. However, the high complexity of the organoid structure and heterogeneity of cellular composition bring challenges associated with cryoprotectant distribution, distinct response of cells to the solution and freezing-induced injuries. The deficit of adequate quality control methods, which may ensure the assessment of organoid recovery in due term without prolonged re-cultivation process, represents another challenge limiting the reproducibility of current cryobanking technology. In this review, we attempt to assess the current demands and achievements in organoid cryopreservation and highlight the key questions to focus on during the development of organoid preservation technologies. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2024 Electronic address https://doi.org/10.54680/fr23210110112
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