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Effect of heparin as an anticoagulant on measurements of the erythrocyte genome size using flow cytometry in bony fishes of different ploidy
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SYSNO ASEP 0573126 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Effect of heparin as an anticoagulant on measurements of the erythrocyte genome size using flow cytometry in bony fishes of different ploidy Author(s) Halačka, Karel (UBO-W) RID, SAI
Janko, K. (CZ)
Fedorčák, J. (SK)
Koščo, J. (SK)
Vetešník, Lukáš (UBO-W) RID, SAI, ORCIDNumber of authors 5 Source Title Acta veterinaria Brno. - : Veterinární a farmaceutická univerzita Brno - ISSN 0001-7213
Roč. 92, č. 2 (2023), s. 171-179Number of pages 9 s. Language eng - English Country CZ - Czech Republic Keywords C-value ; dapi ; DNA content ; Fish ; Propidium iodide Subject RIV GL - Fishing OECD category Veterinary science Method of publishing Open access Institutional support UBO-W - RVO:68081766 UT WOS 001018962100009 EID SCOPUS 85160319609 DOI 10.2754/avb202392020171 Annotation The aim of the study was to verify whether the use of heparin as an anticoagulant can affect the results of genome size measurements using a flow cytometer. The values obtained by measuring blood samples with different concentrations of heparin were compared. The differences observed were highly significant, suggesting that the results of previous comparative studies, or those determining individual polyploidy, may need reinterpretation. We anticipate that our findings will increase the accuracy of flow cytometric analysis and will be useful for further surveys of genome size undertaken using blood samples. Workplace Institute of Vertebrate Biology Contact Hana Slabáková, slabakova@ivb.cz, Tel.: 543 422 524 Year of Publishing 2024 Electronic address https://actavet.vfu.cz/media/pdf/actavet_2023092020171.pdf
Number of the records: 1