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Development of the Pseudomonas syringae pv. morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber
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SYSNO ASEP 0572880 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Development of the Pseudomonas syringae pv. morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber Author(s) Budil, Jakub (FZU-D) ORCID, RID
Štenclová, Pavla (FZU-D) ORCID
Kromka, Alexander (FZU-D) RID, ORCID, SAI
Lišková, P. (CZ)Number of authors 4 Source Title Applied Spectroscopy. - : Sage - ISSN 0003-7028
Roč. 77, č. 5 (2023), s. 500-512Number of pages 26 s. Language eng - English Country GB - United Kingdom Keywords ATR FT-IR ; infrared attenuated total reflection ; IR-ATR ; Pseudomonas syringae ; biofilm ; confocal laser scanning microscopy ; CLSM ; model Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LM2023051 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob Method of publishing Limited access Institutional support FZU-D - RVO:68378271 UT WOS 000976875300001 EID SCOPUS 85153745281 DOI 10.1177/00037028231165057 Annotation BIn the present study, infrared attenuated total reflection spectroscopy coupled with optical and confocal laser scanning microscopy has been applied for the first time to analyze Pseudomonas syringae pathovar morsprunorum biofilm development in real time. The biofilm development was observed within a spectral window 4000–800 cm−1 under constant flow conditions for 72 h. The kinetics of representative integrated band areas (nucleic acids with polysaccharides at 1141–1006 cm−1, amino acid side chains with free fatty acids at 1420–1380 cm−1, proteins at 1580–1490 cm−1, and lipids with proteins at 2935–2915 cm−1) were analyzed with regard to the observed biofilm structure and the following P. syringae biofilm developmental stages were attributed: The inoculation phase, washing of weakly attached bacteria closely followed by recolonization of the vacated surface, the restructuration phase, and finally the maturation phase. Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2024 Electronic address https://doi.org/10.1177/00037028231165057
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