Number of the records: 1  

Development of the Pseudomonas syringae pv. morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber

  1. 1.
    SYSNO ASEP0572880
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleDevelopment of the Pseudomonas syringae pv. morsprunorum Biofilm Monitored in Real Time Using Attenuated Total Reflection Fourier Transform Infrared Measurements in a Flow Cell Chamber
    Author(s) Budil, Jakub (FZU-D) ORCID, RID
    Štenclová, Pavla (FZU-D) ORCID
    Kromka, Alexander (FZU-D) RID, ORCID, SAI
    Lišková, P. (CZ)
    Number of authors4
    Source TitleApplied Spectroscopy. - : Sage - ISSN 0003-7028
    Roč. 77, č. 5 (2023), s. 500-512
    Number of pages26 s.
    Languageeng - English
    CountryGB - United Kingdom
    KeywordsATR FT-IR ; infrared attenuated total reflection ; IR-ATR ; Pseudomonas syringae ; biofilm ; confocal laser scanning microscopy ; CLSM ; model
    Subject RIVEE - Microbiology, Virology
    OECD categoryMicrobiology
    R&D ProjectsLM2023051 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    EF16_019/0000760 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    Research Infrastructuree-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob
    Method of publishingLimited access
    Institutional supportFZU-D - RVO:68378271
    UT WOS000976875300001
    EID SCOPUS85153745281
    DOI10.1177/00037028231165057
    AnnotationBIn the present study, infrared attenuated total reflection spectroscopy coupled with optical and confocal laser scanning microscopy has been applied for the first time to analyze Pseudomonas syringae pathovar morsprunorum biofilm development in real time. The biofilm development was observed within a spectral window 4000–800 cm−1 under constant flow conditions for 72 h. The kinetics of representative integrated band areas (nucleic acids with polysaccharides at 1141–1006 cm−1, amino acid side chains with free fatty acids at 1420–1380 cm−1, proteins at 1580–1490 cm−1, and lipids with proteins at 2935–2915 cm−1) were analyzed with regard to the observed biofilm structure and the following P. syringae biofilm developmental stages were attributed: The inoculation phase, washing of weakly attached bacteria closely followed by recolonization of the vacated surface, the restructuration phase, and finally the maturation phase.
    WorkplaceInstitute of Physics
    ContactKristina Potocká, potocka@fzu.cz, Tel.: 220 318 579
    Year of Publishing2024
    Electronic addresshttps://doi.org/10.1177/00037028231165057
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.