Detailed Investigation of Human Galectins -1 and -3 by employing selectively deoxyfluorinated N-acetyllactosamines

0572641 - ÚCHP 2024 CZ eng K - Conference Paper (Czech conference)
Kurfiřt, Martin
Detailed Investigation of Human Galectins -1 and -3 by employing selectively deoxyfluorinated N-acetyllactosamines.
Proceedings of Abstracts. Prague: Institute of Chemical Process Fundamentals of the CAS, v. v. i., 2023 - (Bendová, M.; Wagner, Z.; Windell, L.), s. 11-12. ISBN 978-80-86186-29-0.
[Bažant postraguate conference 2023. Prague (CZ), 31.05.2023-01.06.2023]
R&D Projects: GA ČR(CZ) GA23-06115S; GA MŠMT(CZ) LTC20052
Grant - others:MEYS(XE) CA18103
Institutional support: RVO:67985858
Keywords : galectins * NMR techniques * pharmaceutic industry
OECD category: Organic chemistry

This work is focused on a detailed investigation of supramolecular recognition events between human galectins -1 and -3 and monodeoxyfluorinated N-acetyllactosamine probes, which were previously prepared in our laboratory. The recognition was studied using a combination of X-ray crystallography, isothermal titration calorimetry and several advanced NMR techniques, such as 19F T2-filter, 1H–1H STD, 1H–15N CSP or 19F EXSY. These techniques provided insight into the thermodynamics and kinetics of recognition and enabled us to uncover of subtle differences between both investigated galectins. Such differences could be potentially exploited in the selective targeting of individual galectins with important implications for the galectins-focused
pharmaceutic industry.
Permanent Link: https://hdl.handle.net/11104/0343242