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Metabarcoding of soil environmental DNA to estimate plant diversity globally
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SYSNO ASEP 0572140 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Metabarcoding of soil environmental DNA to estimate plant diversity globally Author(s) Vasar, M. (EE)
Davison, J. (EE)
Moora, M. (EE)
Sepp, S. (EE)
Anslan, S. (EE)
Al-Quraishy, S. (SA)
Bahram, M. (EE)
Bueno, C. G. (EE)
Cantero, J. J. (AR)
Fabiano, E. C. (NA)
Decocq, G. (FR)
Drenkhan, T. (EE)
Fraser, L. (CA)
Oja, J. (EE)
Garibay-Orijel, R. (MX)
Hiiesalu, I. (EE)
Koorem, K. (EE)
Mucina, L. (AU)
Öpik, M. (EE)
Pölme, S. (EE)
Pärtel, M. (EE)
Phosri, C. (TH)
Semchenko, M. (EE)
Vahter, T. (EE)
Doležal, Jiří (BU-J) RID, ORCID
Vasco-Palacios, A. M. (CO)
Tedersoo, L. (EE)
Zobel, M. (EE)Article number 1106617 Source Title Frontiers in Plant Science. - : Frontiers Research Foundation - ISSN 1664-462X
Roč. 14, APR 18 2023 (2023)Number of pages 9 s. Language eng - English Country CH - Switzerland Keywords Metabarcoding ; soil environmental DNA ; plant diversity globally Subject RIV EF - Botanics OECD category Plant sciences, botany Method of publishing Open access Institutional support BU-J - RVO:67985939 UT WOS 000980078800001 EID SCOPUS 85159908348 DOI 10.3389/fpls.2023.1106617 Annotation Traditional approaches to collecting large-scale biodiversity data pose huge logistical and technical challenges. We assessed how a comparatively simple method based on sequencing a short fragment (P6 loop) of the chloroplast trnL intron from soil environmental DNA (eDNA) characterises global variation in plant diversity and community composition compared with data derived from traditional plant inventory methods. Large-scale plant diversity and community composition patterns revealed by sequencing eDNA from 325 globally distributed soil samples were broadly in accordance with those derived from sources based on empirical (GBIF) or extrapolated plant distribution and diversity data. The success of the eDNA taxonomy assignment, and the overlap of taxon lists between eDNA and GBIF, was greatest at moderate to high latitudes of the northern hemisphere. On average, around half (mean: 51.5% SD 17.6) of local GBIF records were represented in eDNA databases at the species level, depending on the geographic region. Important experimental considerations for plant eDNA studies include using a sampling volume and design to maximise the number of taxa detected and optimising the sequencing depth. However, increasing the coverage of reference sequence databases would yield the most significant improvements in the accuracy of taxonomic assignments made using the P6 loop of the trnL region Workplace Institute of Botany Contact Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8 Year of Publishing 2024 Electronic address https://doi.org/10.3389/fpls.2023.1106617
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