The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70

Mulholland, Catherine; Jestřábová, Ivana; Sett, Arghya; Ondruš, Marek; Sýkorová, Veronika; Manzanares, Carmen Lorena; Šimončík, O.; Muller, P.; Hocek, Michal

doi:https://dx.doi.org/10.1038/s42004-023-00862-0

Number of the records: 1

The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70

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SYSNO ASEP	0571293
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The selection of a hydrophobic 7-phenylbutyl-7-deazaadenine-modified DNA aptamer with high binding affinity for the Heat Shock Protein 70
Author(s)	Mulholland, Catherine (UOCHB-X) Jestřábová, Ivana (UOCHB-X) Sett, Arghya (UOCHB-X) Ondruš, Marek (UOCHB-X)_{ORCID, RID} Sýkorová, Veronika (UOCHB-X)_{ORCID, RID} Manzanares, Carmen Lorena (UOCHB-X) Šimončík, O. (CZ) Muller, P. (CZ) Hocek, Michal (UOCHB-X)_{RID, ORCID}
Article number	65
Source Title	Communications Chemistry. - : Nature Publishing Group - ISSN 2399-3669 Roč. 6, April (2023)
Number of pages	11 s.
Language	eng - English
Country	US - United States
Keywords	cell-SELEX ; nucleotide ; evolution
OECD category	Organic chemistry
R&D Projects	GX20-00885X GA ČR - Czech Science Foundation (CSF)
	EF16_019/0000729 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UOCHB-X - RVO:61388963
UT WOS	000964139800002
EID SCOPUS	85152694501
DOI	10.1038/s42004-023-00862-0
Annotation	Nucleic acids aptamers often fail to efficiently target some proteins because of the hydrophilic character of the natural nucleotides. Here we present hydrophobic 7-phenylbutyl-7-deaadenine-modified DNA aptamers against the Heat Shock Protein 70 that were selected via PEX and magnetic bead-based SELEX. After 9 rounds of selection, the pool was sequenced and a number of candidates were identified. Following initial screening, two modified aptamers were chemically synthesised in-house and their binding affinity analysed by two methods, bio-layer interferometry and fluorescent-plate-based binding assay. The binding affinities of the modified aptamers were compared with that of their natural counterparts. The resulting modified aptamers bound with higher affinity (low nanomolar range) to the Hsp70 than their natural sequence (>5 µM) and hence have potential for applications and further development towards Hsp70 diagnostics or even therapeutics.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Jana Procházková, Tel.: 220 183 418
Year of Publishing	2024
Electronic address	https://doi.org/10.1038/s42004-023-00862-0

Number of the records: 1