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Distinguishing Healthy and Carcinoma Cell Cultures Using Fluorescence Spectra Decomposition with a Genetic-Algorithm-Based Code
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SYSNO ASEP 0570687 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Distinguishing Healthy and Carcinoma Cell Cultures Using Fluorescence Spectra Decomposition with a Genetic-Algorithm-Based Code Author(s) Pospíšilová, M. (CZ)
Kalábová, H. (CZ)
Kuncová, Gabriela (UCHP-M) RID, SAIArticle number 256 Source Title Biosensors. - : MDPI
Roč. 13, č. 2 (2023)Number of pages 12 s. Language eng - English Country CH - Switzerland Keywords steady state fluorescence ; cell suspension auto-fluorescence ; genetic algorithm OECD category Medical engineering Method of publishing Open access Institutional support UCHP-M - RVO:67985858 UT WOS 000938446200001 EID SCOPUS 85149124621 DOI https://doi.org/10.3390/bios13020256 Annotation In this paper, we analysed the steady state fluorescence spectra of cell suspensions containing healthy and carcinoma fibroblast mouse cells, using a genetic-algorithm-spectra-decomposition software (GASpeD). In contrast to other deconvolution algorithms, such as polynomial or linear unmixing software, GASpeD takes into account light scatter. In cell suspensions, light scatter plays an important role as it depends on the number of cells, their size, shape, and coagulation. The measured fluorescence spectra were normalized, smoothed and deconvoluted into four peaks and background. The wavelengths of intensities' maxima of lipopigments (LR), FAD, and free/bound NAD(P)H (AF/AB) of the deconvoluted spectra matched published data. In deconvoluted spectra at pH = 7, the fluorescence intensities of the AF/AB ratio in healthy cells was always higher in comparison to carcinoma cells. In addition, the AF/AB ratio in healthy and carcinoma cells were influenced differently by changes in pH. In mixtures of healthy and carcinoma cells, AF/AB decreases when more than 13% of carcinoma cells are present. Expensive instrumentation is not required, and the software is user friendly. Due to these attributes, we hope that this study will be a first step in the development of new cancer biosensors and treatments with the use of optical fibers. Workplace Institute of Chemical Process Fundamentals Contact Eva Jirsová, jirsova@icpf.cas.cz, Tel.: 220 390 227 Year of Publishing 2024 Electronic address https://www.mdpi.com/2079-6374/13/2/256
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