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Encapsulating melittin from animal venom by finely tuned charge compensation with polymer carriers
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SYSNO ASEP 0570496 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Encapsulating melittin from animal venom by finely tuned charge compensation with polymer carriers Author(s) Černochová, Zulfiya (UMCH-V) RID, ORCID
Lobaz, Volodymyr (UMCH-V) RID, ORCID
Čtveráčková, Lucie (UMCH-V)
Černoch, Peter (UMCH-V) RID, ORCID
Šlouf, Miroslav (UMCH-V) RID, ORCID
Filipová, Marcela (UMCH-V) RID, ORCID
Hrubý, Martin (UMCH-V) RID, ORCID
Pánek, Jiří (UMCH-V) RID, ORCIDArticle number 111996 Source Title European Polymer Journal. - : Elsevier - ISSN 0014-3057
Roč. 190, 25 May (2023)Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords melittin ; encapsulation ; polyanion Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects GA21-04166S GA ČR - Czech Science Foundation (CSF) GC20-15479J GA ČR - Czech Science Foundation (CSF) LM2023053 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support UMCH-V - RVO:61389013 UT WOS 000958643200001 EID SCOPUS 85150444923 DOI 10.1016/j.eurpolymj.2023.111996 Annotation Due to their wide availability, polypeptide/protein venoms produced by certain insects are of high interest as potential active pharmacological ingredients. However, the number of clinical studies on these venoms remains poor. In this paper, we describe an extremely efficient platform to form polyplexes from cationic amphiphilic animal venoms, such as the hemolytic poison melittin. The idea is based on supramolecular melittin cationic charge compensation with nanoparticles (micelles composed of hydrophobic glassy polystyrene core and hydrophilic poly(meth)acrylic acid corona, PMA and PAA acid homopolymers) with high anion charge density at physiological pH. An instant “mix and go” process and efficient melittin complexation with nanoparticles at concentrations down to micrograms per milliliter of PBS solution was achieved and proven by inhibition of melittin-induced hemolysis. We studied in detail how the structural features of the nanoparticles influence their potency, we found that the most important parameter is the number of carboxylates in the polyanion chain (proportional to the molecular weight and length of the anionic poly(meth)acrylic acid block). The poly(meth)acrylic acid-based polymer nanoparticles may be useful as melittin (and possibly other cationic amphiphilic animal venoms) antidotes but also as models for constructing future delivery systems to apply the venoms therapeutically. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2024 Electronic address https://www.sciencedirect.com/science/article/pii/S0014305723001799?via%3Dihub
Number of the records: 1