Capillary Electrophoresis in Food Analysis

0568344 - ÚOCHB 2023 RIV SG eng M - Monography Chapter
Štěpánová, Sille - Kašička, Václav
Analysis of Peptides by Capillary Electromigration Methods.
Capillary Electrophoresis in Food Analysis. Singapore: Bentham Science Publishers, 2022 - (Castro-Puyana, M.; Herrero, M.; Marina, L.), s. 109-146. Current and Future Developments in Food Science, 2. ISBN 978-981-5036-16-9
R&D Projects: GA ČR(CZ) GA18-02597S
Institutional support: RVO:61388963
Keywords : bioactive peptides * capillary electrophoresis * food analysis * peptides in foods
OECD category: Analytical chemistry
https://www.eurekaselect.com/chapter/17194

Peptides themselves and especially as products of enzymatic or chemical cleavage of parental proteins belong to the important components of foodstuffs. They significantly influence their nutritional, biological, technological, and functional properties. Some of these peptides were found to have effects on human health and nutrition, e.g. by affecting human digestive, endocrine, cardiovascular, immune, and nervous systems. Hence, qualitative and quantitative analysis of peptides in foods is of a great importance. For the separation and quantification of peptides in foods, capillary electromigration methods represent one of the most suitable analytical methods. This chapter presents a comprehensive overview of the developments and applications of high performance capillary and microchip electromigration methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography and electrochromatography) for separation and analysis of peptides in foods and food products since 2010 up to middle of 2020. Various aspects of application of capillary electromigration methods for peptide analysis in foods, such as sample preparation, peptide preseparation, preconcentration, derivatization, adsorption suppression, and detection, are described and discussed. Several particular applications of capillary electromigration methods for separation and analysis of peptides in various food samples of animal, plant, and microbial origin are demonstrated.
Permanent Link: https://hdl.handle.net/11104/0339669