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Pedomonas mirosovicensis gen. nov., sp. nov., a bacterium isolated from soil with the aid of Micrococcus luteus culture supernatant containing resuscitation-promoting factor
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SYSNO ASEP 0565771 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Pedomonas mirosovicensis gen. nov., sp. nov., a bacterium isolated from soil with the aid of Micrococcus luteus culture supernatant containing resuscitation-promoting factor Author(s) Lopez Marin, M.A. (CZ)
Šuman, J. (CZ)
Jani, K. (CZ)
Ulbrich, P. (CZ)
Cajthaml, Tomáš (MBU-M) RID, ORCID
Pajer, P. (CZ)
Wolf, J. (DE)
Neumann-Schaal, M. (DE)
Strejček, M. (CZ)
Uhlík, O. (CZ)Article number 005467 Source Title International Journal of Systematic and Evolutionary Microbiology. - : Microbiology Society - ISSN 1466-5026
Roč. 72, č. 8 (2022)Number of pages 12 s. Language eng - English Country GB - United Kingdom Keywords compost soil ; extracellular organic matter ; resuscitation-promoting factor ; soil bacteria ; Sphingosinicellaceae ; whole genome average nucleotide identity Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects LM2018131 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000888667900006 EID SCOPUS 85135312881 DOI 10.1099/ijsem.0.005467 Annotation An orange-golden iridescent culture, designated A1X5R2(T), was isolated from a compost soil suspension which was amended with Micrococcus luteus NCTC 2665(T) culture supernatant. The cells were non-motile, Gram-stain-negative, 0.4-0.5 mu m wide and 0.7-1.4 mu m long. The 16S rRNA-based phylogenetic and whole-genome analyses revealed that strain A1X5R2(T) forms a distinct lineage within the family Sphingosinicellaceae and is closely related to members of the genus Sphingoaurantiacus ( S. capsulatus , 93.04 % similarity, and S. polygranulatus , 92.77 %). The organism grew at 22-47 degrees C (optimal at 37 degrees C), salinity <3 % (optimal at 1.5 %) and at pH 7. The major respiratory quinone was ubiquinone-10, but a small quantity of ubiquinone-9 was also detected The major polyamine was homospermidine, but a small quantity of putrescine was also detected. The strain contained C-18:1 omega 7c, C-16:0, C-16:1 omega 7c and C-18:0 as the major fatty acids. The main polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol, sphingoglycolipid, diphosphatidylglycerol, two unidentified phospholipids and three unidentified amino lipids. The DNA G+C content was 64.9 mol%. According to the results of phylogenetic and phylogenomic analyses, as well as its physiological characteristics, strain A2X5R2(T) represents the type species of a novel genus within the family Sphingosinicellaceae . The name Pedomonas mirosovicensis gen. nov., sp. nov. is proposed, with the type strain being A1X5R2(T) (=NCCB 100839(T)=DSM 112829(T)). Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005467
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