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High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing
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SYSNO ASEP 0564938 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title High-throughput interspecies profiling of acidic plant hormones using miniaturised sample processing Author(s) Široká, Jitka (UEB-Q) ORCID
Brunoni, Federica (UEB-Q) ORCID
Pěnčík, Aleš (UEB-Q) ORCID, RID, SAI
Mik, V. (CZ)
Žukauskaitė, A. (CZ)
Strnad, Miroslav (UEB-Q) RID, ORCID
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
Floková, Kristýna (UEB-Q) ORCID, RIDNumber of authors 8 Article number 122 Source Title Plant Methods. - : BioMed Central
Roč. 18, č. 1 (2022)Number of pages 15 s. Language eng - English Country GB - United Kingdom Keywords 3D printing ; Evolutionarily distant plant species ; High-throughput ; In-tip microSPE ; Liquid chromatography ; Mass spectrometry ; Miniaturisation ; Plant hormones OECD category Plant sciences, botany Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000884734300001 EID SCOPUS 85142178053 DOI 10.1186/s13007-022-00954-3 Annotation Background: Acidic phytohormones are small molecules controlling many physiological functions in plants. A comprehensive picture of their profiles including the active forms, precursors and metabolites provides an important insight into ongoing physiological processes and is essential for many biological studies performed on plants. Results: A high-throughput sample preparation method for liquid chromatography–tandem mass spectrometry determination of 25 acidic phytohormones classed as auxins, jasmonates, abscisates and salicylic acid was optimised. The method uses a small amount of plant tissue (less than 10 mg fresh weight) and acidic extraction in 1 mol/L formic acid in 10% aqueous methanol followed by miniaturised purification on reverse phase sorbent accommodated in pipette tips organised in a 3D printed 96-place interface, capable of processing 192 samples in one run. The method was evaluated in terms of process efficiency, recovery and matrix effects as well as establishing validation parameters such as accuracy and precision. The applicability of the method in relation to the amounts of sample collected from distantly related plant species was evaluated and the results for phytohormone profiles are discussed in the context of literature reports. Conclusion: The method developed enables high-throughput profiling of acidic phytohormones with minute amounts of plant material, and it is suitable for large scale interspecies studies. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2023 Electronic address https://doi.org/10.1186/s13007-022-00954-3
Number of the records: 1