Quaternized chitosan/heparin polyelectrolyte multilayer films for protein delivery

Urbaniak, Tomasz; García-Briones, Gabriela S.; Zhigunov, Alexander; Hladysh, Sviatoslav; Adrian, Edyta; Lobaz, Volodymyr; Krunclová, Tereza; Janoušková, Olga; Pop-Georgievski, Ognen; Kubies, Dana

doi:https://dx.doi.org/10.1021/acs.biomac.2c00926

Number of the records: 1

Quaternized chitosan/heparin polyelectrolyte multilayer films for protein delivery

1.

SYSNO ASEP	0563944
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Quaternized chitosan/heparin polyelectrolyte multilayer films for protein delivery
Author(s)	Urbaniak, Tomasz (UMCH-V) García-Briones, Gabriela S. (UMCH-V) Zhigunov, Alexander (UMCH-V)_{RID, ORCID} Hladysh, Sviatoslav (UMCH-V)_{RID, ORCID} Adrian, Edyta (UMCH-V)_{ORCID, RID} Lobaz, Volodymyr (UMCH-V)_{RID, ORCID} Krunclová, Tereza (UMCH-V)_ORCID Janoušková, Olga (UMCH-V)_{RID, SAI, ORCID} Pop-Georgievski, Ognen (UMCH-V)_{RID, ORCID} Kubies, Dana (UMCH-V)_{RID, ORCID}
Source Title	Biomacromolecules. - : American Chemical Society - ISSN 1525-7797 Roč. 23, č. 11 (2022), s. 4734-4748
Number of pages	15 s.
Language	eng - English
Country	US - United States
Keywords	quaternized chitosan ; layer-by-layer film ; protein release
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
R&D Projects	GA20-08679S GA ČR - Czech Science Foundation (CSF)
	LX22NPO5102 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Method of publishing	Open access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000886824100001
EID SCOPUS	85140966930
DOI	10.1021/acs.biomac.2c00926
Annotation	Layer-by-layer (LbL) polyelectrolyte coatings are intensively studied as reservoirs of bioactive proteins for modulating interactions between biomaterial surfaces and cells. Mild conditions for the incorporation of growth factors into delivery systems are required to maintain protein bioactivity. Here, we present LbL films composed of water-soluble N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan chloride (HTCC), heparin (Hep), and tannic acid (TA) fabricated under physiological conditions with the ability to release heparin-binding proteins. Surface plasmon resonance analysis showed that the films formed on an anchoring HTCC/TA bilayer, with TA serving as a physical crosslinker, were more stable during their assembly, leading to increased film thickness and increased protein release. X-ray reflectivity measurements confirmed intermixing of the deposited layers. Protein release also increased when the proteins were present as an integral part of the Hep layers rather than as individual protein layers. The 4-week release pattern depended on the protein type, VEGF, CXCL12, and TGF-β1 exhibited a typical high initial release, whereas FGF-2 was sustainably released over 4 weeks. Notably, the films were nontoxic, and the released proteins retained their bioactivity, as demonstrated by the intensive chemotaxis of T-lymphocytes in response to the released CXCL12. Therefore, the proposed LbL films are promising biomaterial coating candidates for stimulating cellular responses.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2023
Electronic address	https://pubs.acs.org/doi/10.1021/acs.biomac.2c00926

Number of the records: 1