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Microbial utilization of simple and complex carbon compounds in a temperate forest soil
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SYSNO ASEP 0560514 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Microbial utilization of simple and complex carbon compounds in a temperate forest soil Author(s) Martinović, Tijana (MBU-M) ORCID
Mašínová, Tereza (MBU-M) ORCID
López-Mondejár, Rubén (MBU-M) ORCID, RID
Jansa, Jan (MBU-M) RID, ORCID
Štursová, Martina (MBU-M)
Starke, Robert (MBU-M) ORCID, RID
Baldrian, Petr (MBU-M) RID, ORCIDArticle number 108786 Source Title Soil Biology and Biochemistry. - : Elsevier - ISSN 0038-0717
Roč. 173, October 2022 (2022)Number of pages 10 s. Language eng - English Country GB - United Kingdom Keywords Soil ecology ; Fungi ; Bacteria ; Decomposition ; Carbon utilization ; Biopolymers ; Exudates Subject RIV EE - Microbiology, Virology OECD category Microbiology R&D Projects GA22-30769S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support MBU-M - RVO:61388971 UT WOS 000843493300001 EID SCOPUS 85135684009 DOI 10.1016/j.soilbio.2022.108786 Annotation Forest soil processes carried out by microorganisms are critical for the global carbon (C) cycle and climate. Characterizing the utilization of differently recalcitrant C sources is an important step towards understanding the ecosystem-level function of microorganisms in temperate forest soils. Here, using stable-isotope probing (SIP), we tracked C incorporation into bacterial and fungal biomass by quantifying 13C incorporation into phospholipid fatty acids (PLFA-SIP), its respiration (i.e., content in the produced CO2) and C accumulation by individual microbial taxa (DNA-SIP), following the addition of 13C-labelled substrates of different recalcitrance (citric acid, glucose, chitin, cellulose, hemicellulose, and plant biomass) in microcosms. The highest 13C respiration was observed after the addition of the low-molecular-mass substrates citric acid and glucose, while the highest 13C incorporation into microbial biomass was observed during growth on chitin. Communities of fungi and bacteria that incorporated 13C of various origins into their biomass differed from the original soil communities, as well as between treatments. The most distinct microbial community was observed in microcosms containing 13C-chitin, indicating its utilization by both fungi and bacteria. Bacterial taxa were more often versatile, incorporating C of various origins, while there was a higher share of fungi that were specialists. Together, our results show that lowmolecular-mass compounds that belong to typical root exudates are more readily respired, while the C from biopolymers studied was relatively more incorporated into microbial biomass. Various C sources are targeted by distinct microbial communities, although their composition partly overlaps due to the existence of generalist bacteria and fungi that are capable of utilizing various C sources. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.sciencedirect.com/science/article/pii/S0038071722002437?via%3Dihub
Number of the records: 1