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Protein coronas coating polymer-stabilized silver nanocolloids attenuate cytotoxicity with minor effects on antimicrobial performance

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    SYSNO ASEP0560415
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleProtein coronas coating polymer-stabilized silver nanocolloids attenuate cytotoxicity with minor effects on antimicrobial performance
    Author(s) Batista, C. C. S. (BR)
    Panico, K. (BR)
    Trousil, Jiří (UMCH-V) RID, ORCID
    Janoušková, Olga (UMCH-V) RID, SAI, ORCID
    de Castro, C. E. (BR)
    Štěpánek, Petr (UMCH-V) RID, ORCID
    Giacomelli, F. C. (BR)
    Article number112778
    Source TitleColloids and Surfaces B-Biointerfaces. - : Elsevier - ISSN 0927-7765
    Roč. 218, October (2022)
    Number of pages11 s.
    Languageeng - English
    CountryNL - Netherlands
    Keywordsnano-bio interface ; silver colloids ; protein corona
    Subject RIVJJ - Other Materials
    OECD categoryNano-materials (production and properties)
    Method of publishingLimited access
    Institutional supportUMCH-V - RVO:61389013
    UT WOS000860770300006
    EID SCOPUS85136154636
    DOI10.1016/j.colsurfb.2022.112778
    AnnotationSilver nanoparticles are versatile platforms with a variety of applications in the biomedical field. In this framework, their presence in biological media inevitably leads to the interaction with proteins thus conducting to the formation of biomolecular coronas. This feature alters the identity of the nanomaterial and may affect many biological events. These considerations motivated the investigation of protein adsorption onto the surface of polymer-stabilized AgNPs. The metallic colloids were coated by polyethyleneimine (PEI), polyvinylpyrrolidone (PVP), and poly(2-vinyl pyridine)-b-poly(ethylene oxide) (PEO-b-P2VP), and nanoparticle-protein interaction was probed by using a library of analytical techniques. The experimental data revealed a higher extent of protein adsorption at the surface of AgNPs@PVP whereas PEO-b-P2VP coating conducted to the least amount. The main component of the protein coronas was evidenced to be bovine serum albumin (BSA), which is indeed the protein at the highest abundancy in the model biological media. We have further demonstrated reduced cytotoxicity of the silver colloids coated by biomolecular coronas as compared to the pristine counterparts. Nevertheless, the protein coatings did not notably reduce the antimicrobial performance of the polymer-stabilized AgNPs. Accordingly, although the protein-repelling property is frequently targeted towards longer in vivo circulation of nanoparticles, we herein underline that protein coatings, which are commonly treated as artifacts to be avoided, may indeed enhance the biological performance of nanomaterials. These findings are expected to be highly relevant in the design of polymer-stabilized metallic colloids intended to be used in healthcare.
    WorkplaceInstitute of Macromolecular Chemistry
    ContactEva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
    Year of Publishing2023
    Electronic addresshttps://www.sciencedirect.com/science/article/pii/S0927776522004611?via%3Dihub
Number of the records: 1  

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