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Males-females differences in the spectrum of chromosomal aberrations in the group of nanocomposites production workers
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SYSNO ASEP 0560220 Document Type K - Proceedings Paper (Czech conf.) R&D Document Type Conference Paper Title Males-females differences in the spectrum of chromosomal aberrations in the group of nanocomposites production workers Author(s) Rössnerová, Andrea (UEM-P) RID
Pelcová, D. (CZ)
Ždímal, Vladimír (UCHP-M) RID, ORCID, SAI
Elzeinova, Fatima (UEM-P)
Margaryan, Hasmik (UEM-P)
Chvojková, Irena (UEM-P)
Topinka, Jan (UEM-P) RID, ORCID
Schwarz, Jaroslav (UCHP-M) RID, ORCID, SAI
Ondráček, Jakub (UCHP-M) RID, ORCID, SAI
Koštejn, Martin (UCHP-M) RID, SAI, ORCID
Komarc, M. (CZ)
Vlčková, Š. (CZ)
Fenclová, Z. (CZ)
Lischková, L. (CZ)
Dvořáčková, Š. (CZ)
Rössner ml., Pavel (UEM-P) RID, ORCIDSource Title Nanocon 2019 : Proceedings of the International Conference on Nanomaterials - Research & Application /11./. - Ostrava : Tanger Ltd., 2020 / Shrbená-Váňová J. - ISBN 978-80-87294-95-6
S. 502-507Number of pages 6 s. Publication form Print - P Action International Conference on Nanomaterials - Research and Application, NANOCON 2019 /11./ Event date 16.10.2019 - 18.10.2019 VEvent location Brno Country CZ - Czech Republic Event type WRD Language eng - English Country CZ - Czech Republic Keywords DNA damage ; gender ; chromosomal aberrations ; micronuclei ; nanoparticles ; occupational exposure OECD category Occupational health R&D Projects GA18-02079S GA ČR - Czech Science Foundation (CSF) LO1508 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UEM-P - RVO:68378041 ; UCHP-M - RVO:67985858 UT WOS 000664115400086 EID SCOPUS 85097182983 DOI 10.37904/nanocon.2019.8589 Annotation An increase in the use of nanomaterials (NM) has been witnessed in many areas of human life. Therefore, assessment of genotoxicity of NM and nanoparticles (NP) is one of the main objectives of genetic toxicology. Despite this fact, human cytogenetic studies following the exposure to NP are still rare. Moreover, no relevant information on possible differences in sensitivity to NP related to gender is available.
In this study we periodically (in September 2016, 2017 and 2018; pre-shift and post-shift each year) analyzed a group of workers (both genders), working long time in nanocomposites research, and matched controls. Aerosol exposure monitoring of particulate matter including nano-sized fractions was carried out during working shift. Micronucleus assay using Human Pan Centromeric probes, was applied to distinguish, besides the frequency of total MN in binucleated cells (BNC), also other types of chromosomal damage (losses and breaks). Moreover, whole-chromosome painting (WCP) for autosome #1 and both gonosomes (X and Y) were applied in third sampling period (2018) with the aim to identify the particular structural and numerical chromosomal aberrations.
Obtained results showed: (i) differences in the risk of exposure to NP related to individual working processes (welding, smelting and machining); (ii) differences in chemical composition of nano-fraction; (iii) no effect of chronic exposure of NP (total MN) opposite to significant effect of acute exposure; (iv) gender-related DNA damage differences (females seem to be more sensitive to chromosomal losses). Additional data from WCP suggested increased frequency of numerical aberrations in gonosomes.Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2022 Electronic address https://www.confer.cz/nanocon/2019/92-males-females-differences-in-the-spectrum-of-chromosomal-aberrations-in-the-group-of-nanocomposites-production-workers
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