Anionically functionalized glycogen encapsulates melittin by multivalent interaction

Zhukouskaya, Hanna; Blanco, P. M.; Černochová, Zulfiya; Čtveráčková, Lucie; Staňo, R.; Pavlova, Ewa; Vetrík, Miroslav; Černoch, Peter; Filipová, Marcela; Šlouf, Miroslav; Štěpánek, M.; Hrubý, Martin; Košovan, P.; Pánek, Jiří

doi:https://dx.doi.org/10.1021/acs.biomac.2c00400

Number of the records: 1

Anionically functionalized glycogen encapsulates melittin by multivalent interaction

1.

SYSNO ASEP	0559765
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Anionically functionalized glycogen encapsulates melittin by multivalent interaction
Author(s)	Zhukouskaya, Hanna (UMCH-V) Blanco, P. M. (CZ) Černochová, Zulfiya (UMCH-V)_{RID, ORCID} Čtveráčková, Lucie (UMCH-V) Staňo, R. (AT) Pavlova, Ewa (UMCH-V)_RID Vetrík, Miroslav (UMCH-V)_{RID, ORCID} Černoch, Peter (UMCH-V)_{RID, ORCID} Filipová, Marcela (UMCH-V)_{RID, ORCID} Šlouf, Miroslav (UMCH-V)_{RID, ORCID} Štěpánek, M. (CZ) Hrubý, Martin (UMCH-V)_{RID, ORCID} Košovan, P. (CZ) Pánek, Jiří (UMCH-V)_{RID, ORCID}
Source Title	Biomacromolecules. - : American Chemical Society - ISSN 1525-7797 Roč. 23, č. 8 (2022), s. 3371-3382
Number of pages	12 s.
Language	eng - English
Country	US - United States
Keywords	melitin ; glycogen ; polyelectrolyte
Subject RIV	CD - Macromolecular Chemistry
OECD category	Polymer science
R&D Projects	GA19-10429S GA ČR - Czech Science Foundation (CSF)
	GA21-04166S GA ČR - Czech Science Foundation (CSF)
Research Infrastructure	e-INFRA CZ - 90140 - CESNET, zájmové sdružení právnických osob
Method of publishing	Limited access
Institutional support	UMCH-V - RVO:61389013
UT WOS	000823599900001
EID SCOPUS	85134802029
DOI	10.1021/acs.biomac.2c00400
Annotation	We developed acid-functionalized glycogen conjugates as supramolecular carriers for efficient encapsulation and inhibition of a model cationic peptide melittin─the main component of honeybee venom. For this purpose, we synthesized and characterized a set of glycogens, functionalized to various degrees by several different acid groups. These conjugates encapsulate melittin up to a certain threshold amount, beyond which they precipitate. Computer simulations showed that sufficiently functionalized conjugates electrostatically attract melittin, resulting in its efficient encapsulation in a broad pH range around the physiological pH. Hemolytic assays confirmed in vitro that the effective inhibition of melittin’s hemolytic activity occurs for highly functionalized samples, whereas no inhibition is observed when using low-functionalized conjugates. It can be concluded that functional glycogens are promising carriers for cationic molecular cargos or antidotes against animal venoms under conditions, in which suitable properties such as biodegradability and biocompatibility are crucial.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2023
Electronic address	https://pubs.acs.org/doi/10.1021/acs.biomac.2c00400

Number of the records: 1