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Antibiotic-loaded amphiphilic chitosan nanoparticles target macrophages and kill an intracellular pathogen
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SYSNO ASEP 0559145 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Antibiotic-loaded amphiphilic chitosan nanoparticles target macrophages and kill an intracellular pathogen Author(s) Trousil, Jiří (UMCH-V) RID, ORCID
Dal, N.-J. K. (NO)
Fenaroli, F. (NO)
Schlachet, I. (IL)
Kubíčková, P. (CZ)
Janoušková, Olga (UMCH-V) RID, SAI, ORCID
Pavlova, Ewa (UMCH-V) RID
Škorič, M. (CZ)
Trejbalová, Kateřina (UMG-J) RID
Pavliš, O. (CZ)
Sosnik, A. (IL)Article number 2201853 Source Title Small. - : Wiley - ISSN 1613-6810
Roč. 18, č. 28 (2022)Number of pages 16 s. Language eng - English Country DE - Germany Keywords amphiphilic chitosan nanoparticles ; intracellular infections ; levofloxacin OECD category Biomaterials (as related to medical implants, devices, sensors) Subject RIV - cooperation Institute of Macromolecular Chemistry - Biotechnology ; Bionics
Institute of Molecular Genetics - Microbiology, VirologyMethod of publishing Limited access Institutional support UMCH-V - RVO:61389013 ; UMG-J - RVO:68378050 UT WOS 000809630500001 EID SCOPUS 85131720748 DOI 10.1002/smll.202201853 Annotation In this work, levofloxacin (LVX), a third-generation fluoroquinolone antibiotic, is encapsulated within amphiphilic polymeric nanoparticles of a chitosan-g-poly(methyl methacrylate) produced by self-assembly and physically stabilized by ionotropic crosslinking with sodium tripolyphosphate. Non-crosslinked nanoparticles display a size of 29 nm and a zeta-potential of +36 mV, while the crosslinked counterparts display 45 nm and +24 mV, respectively. The cell compatibility, uptake, and intracellular trafficking are characterized in the murine alveolar macrophage cell line MH-S and the human bronchial epithelial cell line BEAS-2B in vitro. Internalization events are detected after 10 min and the uptake is inhibited by several endocytosis inhibitors, indicating the involvement of complex endocytic pathways. In addition, the nanoparticles are detected in the lysosomal compartment. Then, the antibacterial efficacy of LVX-loaded nanoformulations (50% w/w drug content) is assessed in MH-S and BEAS-2B cells infected with Staphylococcus aureus and the bacterial burden is decreased by 49% and 46%, respectively. In contrast, free LVX leads to a decrease of 8% and 5%, respectively, in the same infected cell lines. Finally, intravenous injection to a zebrafish larval model shows that the nanoparticles accumulate in macrophages and endothelium and demonstrate the promise of these amphiphilic nanoparticles to target intracellular infections.
Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2023 Electronic address https://onlinelibrary.wiley.com/doi/10.1002/smll.202201853
Number of the records: 1