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Fractionation of the water insoluble part of the heterotrophic mutant green microalga Parachlorella kessleri HY1 (Chlorellaceae) biomass: Identification and structure of polysaccharides
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SYSNO ASEP 0558834 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Fractionation of the water insoluble part of the heterotrophic mutant green microalga Parachlorella kessleri HY1 (Chlorellaceae) biomass: Identification and structure of polysaccharides Author(s) Sushytskyi, L. (CZ)
Synytsya, A. (CZ)
Mirzayeva, T. (CZ)
Kalouskova, T. (CZ)
Bleha, R. (CZ)
Čopiková, J. (CZ)
Kubáč, David (MBU-M) RID, ORCID
Grivalský, Tomáš (MBU-M) ORCID
Ulbrich, P. (CZ)
Kaštánek, P. (CZ)Source Title International Journal of Biological Macromolecules. - : Elsevier - ISSN 0141-8130
Roč. 213, July 31 (2022), s. 27-42Number of pages 16 s. Language eng - English Country GB - United Kingdom Keywords cell-wall polysaccharides ; fourier-transform ir ; ft-ir ; chitin ; vulgaris ; spectroscopy ; components ; waste ; oligosaccharides ; chlorophyta ; Green microalgae ; Parachlorella kessleri ; Structural polysaccharides ; Cell wall structure Subject RIV CD - Macromolecular Chemistry OECD category Polymer science R&D Projects FV10155 GA MPO - Ministry of Industry and Trade (MPO) Method of publishing Limited access Institutional support MBU-M - RVO:61388971 UT WOS 000812321000004 EID SCOPUS 85131083302 DOI 10.1016/j.ijbiomac.2022.05.108 Annotation The water-insoluble part of Parachlorella kessleri HY1 biomass was subjected to the extraction of cell-wall polysaccharides using polar aprotic solvents (DMSO, LiCl/DMSO) and aqueous alkaline solutions (0.1, 1 and 4 mol.l(- 1) of NaOH). Proteins predominated in all the crude extracts and in the insoluble residues were partially removed by treatment with proteolytic enzymes (pepsin and pronase), and in some cases with the HCl/H2O2 reagent, yielding purified polysaccharide-enriched fractions. These treatments led to the solubilisation of some products in water. The composition and structure of isolated polysaccharides were characterised based on monosaccharide composition, glycosidic linkage and spectroscopic analyses. The DMSO extract contained mainly proteins, and polysaccharides were not detected. The water-soluble parts isolated from the LiCl/DMSO extract contained alpha-L-rhamnan, alpha-D-glucan and beta-D-glucogalactan, the water-insoluble part contained (1> 4)-beta-D-xylan, first isolated from the biomass of green microalgae. The alkali extracts contained polysaccharides of similar structure, and also water-insoluble (1> 4)-beta-D-mannan. The insoluble part after all extractions contained alpha-chitin as the main polysaccharide, which was confirmed by spectroscopic methods. All these polysaccharides can play a certain role in the cell wall structure of this microalga. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2023 Electronic address https://www.sciencedirect.com/science/article/pii/S0141813022010820?via%3Dihub
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