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Singlet oxygen in vivo: it is all about intensity
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SYSNO ASEP 0557755 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Singlet oxygen in vivo: it is all about intensity Author(s) Hackbarth, S. (DE)
Islam, R. (JP)
Šubr, Vladimír (UMCH-V) RID, ORCID
Etrych, Tomáš (UMCH-V) RID, ORCID
Fang, J. (JP)Article number 891 Source Title Journal of Personalized Medicine. - : MDPI
Roč. 12, č. 6 (2022)Number of pages 11 s. Language eng - English Country CH - Switzerland Keywords photodynamic therapy ; singlet oxygen ; time-resolved phosphorescence Subject RIV CD - Macromolecular Chemistry OECD category Polymer science Method of publishing Open access Institutional support UMCH-V - RVO:61389013 UT WOS 000815913600001 EID SCOPUS 85131434537 DOI 10.3390/jpm12060891 Annotation The presented work addresses the influence of illumination intensity on the amount and locations of singlet oxygen generation in tumor tissue. We used time-resolved optical detection at the typical emission wavelength around 1270 nm and at 1200 nm where there is no singlet oxygen phosphorescence to determine the phosphorescence kinetics. The discussed data comprise in vivo measurements in tumor-laden HET-CAM and mice. The results show that illumination that is too intense is a major issue, affecting many PDT treatments and all singlet oxygen measurements in vivo so far. In such cases, photosensitization and oxygen consumption exceed oxygen supply, limiting singlet oxygen generation to the blood vessels and walls, while photosensitizers in the surrounding tissue will likely not participate. Being a limitation for the treatment, on one hand, on the other, this finding offers a new method for tumor diagnosis when using photosensitizers exploiting the EPR effect. In contrast to high-intensity PDT, some papers reported successful treatment with nanoparticular drugs using much lower illumination intensity. The question of whether, with such illumination, singlet oxygen is indeed generated in areas apart from vessels and walls, is addressed by numerical analysis. In addition, we discuss how to perform measurements at such low intensities. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2023 Electronic address https://www.mdpi.com/2075-4426/12/6/891
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