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The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations
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SYSNO ASEP 0557670 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Potential Role of SP-G as Surface Tension Regulator in Tear Film: From Molecular Simulations to Experimental Observations Author(s) Schicht, M. (DE)
Riedlová, Kamila (UFCH-W) ORCID, RID, SAI
Kukulka, M. (PL)
Li, W. (DE)
Scheer, A. (DE)
Garreis, F. (DE)
Jacobi, Ch. (DE)
Paulsen, F. (DE)
Cwiklik, Lukasz (UFCH-W) RID, ORCID
Bräuer, L. (DE)Article number 5783 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 23, č. 10 (2022)Number of pages 16 s. Language eng - English Country CH - Switzerland Keywords tear film ; surfactant protein ; surface tension Subject RIV CF - Physical ; Theoretical Chemistry OECD category Physical chemistry R&D Projects GA21-19854S GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support UFCH-W - RVO:61388955 UT WOS 000801351400001 EID SCOPUS 85130307178 DOI 10.3390/ijms23105783 Annotation The ocular surface is in constant interaction with the environment and with numerous pathogens. Therefore, complex mechanisms such as a stable tear film and local immune defense mechanisms are required to protect the eye. This study describes the detection, characterization, and putative role of surfactant protein G (SP-G/SFTA2) with respect to wound healing and surface activity. Bioinformatic, biochemical, and immunological methods were combined to elucidate the role of SP-G in tear film. The results show the presence of SP-G in ocular surface tissues and tear film (TF). Increased expression of SP-G was demonstrated in TF of patients with dry eye disease (DED). Addition of recombinant SP-G in combination with lipids led to an accelerated wound healing of human corneal cells as well as to a reduction of TF surface tension. Molecular modeling of TF suggest that SP-G may regulate tear film surface tension and improve its stability through specific interactions with lipids components of the tear film. In conclusion, SP-G is an ocular surface protein with putative wound healing properties that can also reduce the surface tension of the tear film. View Full-Text Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2023 Electronic address http://hdl.handle.net/11104/0331578
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