Number of the records: 1

IMG Electron Microscopy Core Facility

SYSNO ASEP	0555851
Document Type	O - Others
R&D Document Type	Others
Title	IMG Electron Microscopy Core Facility
Author(s)	Filimonenko, Vlada (UMG-J)_{RID, ORCID} Pinkas, Dominik (UMG-J)_ORCID Vlčák, Erik (UMG-J) Raabová, Helena (UMG-J) Hozák, Pavel (UMG-J)_{RID, ORCID}
Year of issue	2021
Language	eng - English
Country	CZ - Czech Republic
Keywords	Transmission lectron microscopy ; sample preparation ; Czech-BioImaging ; Euro-BioImgaging
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Cell biology
R&D Projects	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF18_046/0016045 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	TE01020118 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
Research Infrastructure	Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
Institutional support	UMG-J - RVO:68378050
Annotation	The Electron Microscopy Core Facility provides expertise and cutting-edge equipment for a broad range of biological sample preparation and ultrastructural imaging techniques. The core facility deals with various biological samples: human and animal cell cultures, plant and animal tissues, worms, microorganisms, lipid micelles, isolated DNA. The sample preparation techniques include routine chemical fixation and resin embedding, negative staining, cryofixation using plunge-freezing or high-pressure freezing, freeze-substitution, cryosectioning, freeze fracture replica labeling, pre- and post-embedding immunolabeling, the list being lately extended by cryoCLEM using specialized microscope. High-pressure freezer, two automatic freeze-substitution machines, cryo-ultramicrotomes, automated plunge-freezer, freeze-fracture replica making device, cryoCLEM microscope, as well as additional wet lab equipment are available. Transmission electron microscopes (TEM) installed in the end of 2019 include a standard 120 kV instrument for routine observation and an advanced 200 kV S/TEM providing the possibility of high-resolution TEM, 3D electron tomography, cryoelectron microscopy and EDS elemental analysis. The team has a long expertise in the development and optimization of sample preparation techniques, including fruitful collaborations with companies providing equipment for electron microscopy. The Electron Microscopy Core Facility is part of the IMG Czech-BioImaging node and Prague Euro-BioImaging node. We provide open access to our technologies and expertise and are ready to welcome users from all fields.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2022

Number of the records: 1