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Nanoaggregate-forming lipid-conjugated AS1411 aptamer as a promising tumor-targeted delivery system of anticancer agents in vitro'
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SYSNO ASEP 0555750 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nanoaggregate-forming lipid-conjugated AS1411 aptamer as a promising tumor-targeted delivery system of anticancer agents in vitro' Author(s) Carvalho, J. (PT)
Lopes-Nunes, J. (PT)
Vialet, B. (FR)
Rosado, T. (PT)
Gallardo, E. (PT)
Vale, J. (PT)
Eloy, C. (PT)
Ferreira, S. (PT)
Palmeira-de-Oliveira, R. (PT)
Campello, M. P. C. (PT)
Paulo, A. (PT)
Barthelemy, P. (FR)
Mergny, Jean-Louis (BFU-R) ORCID, RID
Salgado, G.F. (FR)
Queiroz, J.A. (PT)
Ellington, A. (US)
Cruz, C. (PT)Number of authors 17 Article number 102429 Source Title Nanomedicine: Nanotechnology, Biology and Medicine. - : Elsevier - ISSN 1549-9634
Roč. 36, AUG 2021 (2021)Number of pages 13 s. Publication form Online - E Language eng - English Country US - United States Keywords g-quadruplex ; surfactants ; micelle ; sirna ; Nanoaggregates ; Drug delivery ; AS1411 G-quadruplex ; Cervical cancer ; hpv OECD category Other medical science R&D Projects EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000687935400013 EID SCOPUS 85109880646 DOI 10.1016/j.nano.2021.102429 Annotation Nanoparticles offer targeted delivery of drugs with minimal toxicity to surrounding healthy tissue and have great potential in the management of human papillomavirus (HPV)-related diseases. We synthesized lipid-modified AS1411 aptamers capable of forming nanoaggregates in solution containing Mg2+. The nanoaggregates presented suitable properties for pharmaceutical applications such as small size (100 nm), negative charge, and drug release. The nanoaggregates were loaded with acridine orange derivative C-8 for its specific delivery into cervical cancer cell lines and HPV-positive tissue biopsies. This improved inhibition of HeLa proliferation and cell uptake without significantly affecting healthy cells. Finally, the nanoaggregates were incorporated in a gel formulation with promising tissue retention properties aiming at developing a local delivery strategy of the nanoaggregates in the female genital tract. Collectively, these findings suggest that the nanolimnulation protocol has great potential for the delivery of both anticancer and antiviral agents, becoming a novel modality for cervical cancer management. (C) 2021 Elsevier Inc. All rights reserved. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S1549963421000721?via%3Dihub
Number of the records: 1