Number of the records: 1
Incidence of Phage Capsid Organization on the Resistance to High Energy Proton Beams
- 1.
SYSNO ASEP 0554843 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Incidence of Phage Capsid Organization on the Resistance to High Energy Proton Beams Author(s) De Plano, L. M. (IT)
Silipigni, L. (IT)
Torrisi, L. (IT)
Torrisi, A. (IT)
Cutroneo, Mariapompea (UJF-V) ORCID, RID, SAI
Havránek, Vladimír (UJF-V) RID, SAI, ORCID
Macková, Anna (UJF-V) RID, ORCID, SAI
Zammuto, V. (IT)
Gugliandolo, C. (IT)
Rizzo, M. G. (IT)
Guglielmino, S. (IT)
Franco, D. (IT)Number of authors 12 Article number 988 Source Title Applied Sciences-Basel. - : MDPI
Roč. 12, č. 3 (2022)Number of pages 12 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords M13 bacteriophages ; engineered phage clones ; proton beam radiation ; resistance to particles radiation OECD category Atomic, molecular and chemical physics (physics of atoms and molecules including collision, interaction with radiation, magnetic resonances, Mössbauer effect) R&D Projects EF16_013/0001812 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Research Infrastructure CANAM II - 90056 - Ústav jaderné fyziky AV ČR, v. v. i. Method of publishing Open access Institutional support UJF-V - RVO:61389005 UT WOS 000759884100001 EID SCOPUS 85123117213 DOI 10.3390/app12030988 Annotation The helical geometry of virus capsid allows simple self-assembly of identical protein subunits with a low request of free energy and a similar spiral path to virus nucleic acid. Consequently, small variations in protein subunits can affect the stability of the entire phage particle. Previously, we observed that rearrangement in the capsid structure of M13 engineered phages affected the resistance to UV-C exposure, while that to H2O2 was mainly ascribable to the amino acids' sequence of the foreign peptide. Based on these findings, in this work, the resistance to accelerated proton beam exposure (5.0 MeV energy) of the same phage clones was determined at different absorbed doses and dose rates. Then, the number of viral particles able to infect and replicate in the natural host, Escherichia coli F+, was evaluated. By comparing the results with the M13 wild-type vector (pC89), we observed that 12III1 phage clones, with the foreign peptide containing amino acids favorable to carbonylation, exhibited the highest reduction in phage titer associated with a radiation damage (RD) of 35 x 10(-3)/Gy at 50 dose Gy. On the other hand, P9b phage clones, containing amino acids unfavorable to carbonylation, showed the lowest reduction with an RD of 4.83 x 10(-3)/Gy at 500 dose Gy. These findings could improve the understanding of the molecular mechanisms underlying the radiation resistance of viruses Workplace Nuclear Physics Institute Contact Markéta Sommerová, sommerova@ujf.cas.cz, Tel.: 266 173 228 Year of Publishing 2023 Electronic address https://doi.org/10.3390/app12030988
Number of the records: 1