A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA(Trp) to decode the UGA codon in Trypanosoma brucei

Paris, Zdeněk; Svobodová, Michaela; Kachale, Ambar; Horáková, Eva; Nenarokova, Anna; Lukeš, Julius

doi:https://dx.doi.org/10.1080/15476286.2021.1940445

Number of the records: 1

A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA(Trp) to decode the UGA codon in Trypanosoma brucei

1.

SYSNO ASEP	0554452
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	A mitochondrial cytidine deaminase is responsible for C to U editing of tRNA(Trp) to decode the UGA codon in Trypanosoma brucei
Author(s)	Paris, Zdeněk (BC-A)_RID Svobodová, Michaela (BC-A)_SAI Kachale, Ambar (BC-A)_{SAI, ORCID} Horáková, Eva (BC-A)_{RID, ORCID} Nenarokova, Anna (BC-A)_{RID, ORCID} Lukeš, Julius (BC-A)_{RID, ORCID}
Number of authors	6
Source Title	RNA biology. - : Taylor & Francis - ISSN 1547-6286 Roč. 18, JUL 2021 (2021), s. 278-286
Number of pages	9 s.
Publication form	Print - P
Language	eng - English
Country	US - United States
Keywords	transfer-rna import ; leishmania-tarentolae ; adenosine-deaminase ; protein-synthesis ; anticodon ; translation ; identification ; thiolation ; stability ; inosine ; Mitochondrion ; tryptophanyl tRNA ; editing ; cytidine deaminase ; trypanosoma
Subject RIV	FP - Other Medical Disciplines
OECD category	Parasitology
R&D Projects	GA20-11585S GA ČR - Czech Science Foundation (CSF)
	GA18-15962S GA ČR - Czech Science Foundation (CSF)
	LL1601 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	EF16_019/0000759 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA20-07186S GA ČR - Czech Science Foundation (CSF)
Method of publishing	Open access
Institutional support	BC-A - RVO:60077344
UT WOS	000669722800001
EID SCOPUS	85109619571
DOI	10.1080/15476286.2021.1940445
Annotation	In kinetoplastid protists, all mitochondrial tRNAs are encoded in the nucleus and imported from the cytoplasm to maintain organellar translation. This also applies to the tryptophanyl tRNA (tRNA(Trp)) encoded by a single-copy nuclear gene, with a CCA anticodon to read UGG codon used in the cytosolic translation. Yet, in the mitochondrion it is unable to decode the UGA codon specifying tryptophan. Following mitochondrial import of tRNA(Trp), this problem is solved at the RNA level by a single C34 to U34 editing event that creates the UCA anticodon, recognizing UGA. To identify the enzyme responsible for this critical editing activity, we scrutinized the genome of Trypanosoma brucei for putative cytidine deaminases as the most likely candidates. Using RNAi silencing and poisoned primer extension, we have identified a novel deaminase enzyme, named here TbmCDAT for mitochondrial Cytidine Deaminase Acting on tRNA, which is responsible for this organelle-specific activity in T. brucei. The ablation of TbmCDAT led to the downregulation of mitochondrial protein synthesis, supporting its role in decoding the UGA tryptophan codon.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2022
Electronic address	https://www.tandfonline.com/doi/full/10.1080/15476286.2021.1940445

Number of the records: 1