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A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface
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SYSNO ASEP 0554407 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface Author(s) Hesko, Ondřej (BFU-R)
Fojta, Miroslav (BFU-R) RID, ORCID
Špaček, Jan (BFU-R) ORCIDNumber of authors 3 Article number 115773 Source Title Journal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
Roč. 901, NOV 15 2021 (2021)Number of pages 6 s. Publication form Online - E Language eng - English Country CH - Switzerland Keywords electrochemical oxidation ; nucleic-acids ; oligonucleotides ; dna ; voltammetry Subject RIV CG - Electrochemistry OECD category Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis) R&D Projects EF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA20-03187S GA ČR - Czech Science Foundation (CSF) Method of publishing Limited access Institutional support BFU-R - RVO:68081707 UT WOS 000714137900001 EID SCOPUS 85117389455 DOI 10.1016/j.jelechem.2021.115773 Annotation Previously it has been shown that cyclic nucleoside monophosphates can spontaneously polymerize to form RNA oligonucleotides under conditions simulating prebiotic conditions on Archean Earth. The most efficient polymerization was documented with 3',5'-cyclic guanosine monophosphate (cGMP). In this work a method for fast detection of short polyG RNAs present in a large overabundance of cGMP, modeling conditions in the non-enzymatic nucleotide polymerization mixtures, is presented. The method is based on electrochemical measurements of guanine (G) oxidation signals yielded by RNA oligomers adsorbed onto the surface of a pyrolytic graphite electrode (PGE). To avoid false positive results arising from the G oxidation signals due to coadsorbed cGMP, a method for selective removal of the monomers from the electrode surface has been devised. In the first step, both cGMP and RNAs are co-adsorbed onto the PGE surface. In the second step, the cGMP is selectively desorbed using treatments in solutions of different tested surfactants (SDS, Tween 20 or Triton X100), or by washing in deionized water at elevated temperature. We show that this new approach is suitable for selective analysis of products of polymerization reactions from mixtures of their building blocks. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2022 Electronic address https://www.sciencedirect.com/science/article/pii/S1572665721007992?via%3Dihub
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