Number of the records: 1  

A simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface

  1. 1.
    SYSNO ASEP0554407
    Document TypeJ - Journal Article
    R&D Document TypeJournal Article
    Subsidiary JČlánek ve WOS
    TitleA simple electroanalysis of polyG RNA in mixtures with 3',5'-cyclic guanosine monophosphate achieved by selective desorption of the monomers from the electrode surface
    Author(s) Hesko, Ondřej (BFU-R)
    Fojta, Miroslav (BFU-R) RID, ORCID
    Špaček, Jan (BFU-R) ORCID
    Number of authors3
    Article number115773
    Source TitleJournal of Electroanalytical Chemistry. - : Elsevier - ISSN 1572-6657
    Roč. 901, NOV 15 2021 (2021)
    Number of pages6 s.
    Publication formOnline - E
    Languageeng - English
    CountryCH - Switzerland
    Keywordselectrochemical oxidation ; nucleic-acids ; oligonucleotides ; dna ; voltammetry
    Subject RIVCG - Electrochemistry
    OECD categoryElectrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
    R&D ProjectsEF15_003/0000477 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
    GA20-03187S GA ČR - Czech Science Foundation (CSF)
    Method of publishingLimited access
    Institutional supportBFU-R - RVO:68081707
    UT WOS000714137900001
    EID SCOPUS85117389455
    DOI10.1016/j.jelechem.2021.115773
    AnnotationPreviously it has been shown that cyclic nucleoside monophosphates can spontaneously polymerize to form RNA oligonucleotides under conditions simulating prebiotic conditions on Archean Earth. The most efficient polymerization was documented with 3',5'-cyclic guanosine monophosphate (cGMP). In this work a method for fast detection of short polyG RNAs present in a large overabundance of cGMP, modeling conditions in the non-enzymatic nucleotide polymerization mixtures, is presented. The method is based on electrochemical measurements of guanine (G) oxidation signals yielded by RNA oligomers adsorbed onto the surface of a pyrolytic graphite electrode (PGE). To avoid false positive results arising from the G oxidation signals due to coadsorbed cGMP, a method for selective removal of the monomers from the electrode surface has been devised. In the first step, both cGMP and RNAs are co-adsorbed onto the PGE surface. In the second step, the cGMP is selectively desorbed using treatments in solutions of different tested surfactants (SDS, Tween 20 or Triton X100), or by washing in deionized water at elevated temperature. We show that this new approach is suitable for selective analysis of products of polymerization reactions from mixtures of their building blocks.
    WorkplaceInstitute of Biophysics
    ContactJana Poláková, polakova@ibp.cz, Tel.: 541 517 244
    Year of Publishing2022
    Electronic addresshttps://www.sciencedirect.com/science/article/pii/S1572665721007992?via%3Dihub
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.