The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus

Balaban, Can; Sztacho, Martin; Blažíková, Michaela; Hozák, Pavel

doi:https://dx.doi.org/10.3390/cells10040848

Number of the records: 1

The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus

1.

SYSNO ASEP	0554294
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The F-Actin-Binding MPRIP Forms Phase-Separated Condensates and Associates with PI(4,5)P2 and Active RNA Polymerase II in the Cell Nucleus
Author(s)	Balaban, Can (UMG-J) Sztacho, Martin (UMG-J)_ORCID Blažíková, Michaela (UMG-J) Hozák, Pavel (UMG-J)_{RID, ORCID}
Number of authors	4
Article number	848
Source Title	Cells. - : MDPI Roč. 10, č. 4 (2021)
Number of pages	17 s.
Publication form	Online - E
Language	eng - English
Country	CH - Switzerland
Keywords	mprip ; phase separation ; pip2 ; actin ; nucleus
Subject RIV	EB - Genetics ; Molecular Biology
OECD category	Cell biology
R&D Projects	GA19-05608S GA ČR - Czech Science Foundation (CSF)
	GA18-19714S GA ČR - Czech Science Foundation (CSF)
	EF16_013/0001775 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	ED1.1.00/02.0109 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTC19048 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LTC20024 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Research Infrastructure	Czech-BioImaging - 90062 - Ústav molekulární genetiky AV ČR, v. v. i. Czech-BioImaging II - 90129 - Ústav molekulární genetiky AV ČR, v. v. i.
Method of publishing	Open access
Institutional support	UMG-J - RVO:68378050
UT WOS	000642893600001
DOI	10.3390/cells10040848
Annotation	Here, we provide evidence for the presence of Myosin phosphatase rho-interacting protein (MPRIP), an F-actin-binding protein, in the cell nucleus. The MPRIP protein binds to Phosphatidylinositol 4,5-bisphosphate (PIP2) and localizes to the nuclear speckles and nuclear lipid islets which are known to be involved in transcription. We identified MPRIP as a component of RNA Polymerase II/Nuclear Myosin 1 complex and showed that MPRIP forms phase-separated condensates which are able to bind nuclear F-actin fibers. Notably, the fibrous MPRIP preserves its liquid-like properties and reforms the spherical shaped condensates when F-actin is disassembled. Moreover, we show that the phase separation of MPRIP is driven by its long intrinsically disordered region at the C-terminus. We propose that the PIP2/MPRIP association might contribute to the regulation of RNAPII transcription via phase separation and nuclear actin polymerization.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2022
Electronic address	https://www.mdpi.com/2073-4409/10/4/848

Number of the records: 1