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Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (x Triticosecale Wittm.) after 5-azacytidine treatment
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SYSNO ASEP 0553335 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparative proteomic analysis provides new insights into regulation of microspore embryogenesis induction in winter triticale (x Triticosecale Wittm.) after 5-azacytidine treatment Author(s) Krzewska, M. (PL)
Dubas, E. (PL)
Gołębiowska, G. (PL)
Nowicka, Anna (UEB-Q) ORCID
Janas, A. (PL)
Zieliński, K. (PL)
Surówka, E. (PL)
Kopeć, P. (PL)
Mielczarek, K. (PL)
Żur, I. (PL)Number of authors 10 Article number 22215 Source Title Scientific Reports. - : Nature Publishing Group - ISSN 2045-2322
Roč. 11, č. 1 (2021)Number of pages 18 s. Language eng - English Country GB - United Kingdom Keywords gene-expression ; stress ; subfamily ; patterns ; anthers ; androgenesis ; barley ; wheat OECD category Plant sciences, botany Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000720624200003 EID SCOPUS 85119048641 DOI 10.1038/s41598-021-01671-y Annotation Effective microspore embryogenesis (ME) requires substantial modifications in gene expression pattern, followed by changes in the cell proteome and its metabolism. Recent studies have awakened also interest in the role of epigenetic factors in microspore de-differentiation and reprogramming. Therefore, demethylating agent (2.5-10 mu M 5-azacytidine, AC) together with low temperature (3 weeks at 4 degrees C) were used as ME-inducing tiller treatment in two doubled haploid (DH) lines of triticale and its effect was analyzed in respect of anther protein profiles, expression of selected genes (TAPETUM DETERMINANT1 (TaTPD1-like), SOMATIC EMBRYOGENESIS RECEPTOR KINASE 2 (SERK2) and GLUTATHIONE S-TRANSFERASE (GSTF2)) and ME efficiency. Tiller treatment with 5.0 mu M AC was the most effective in ME induction, it was associated with (1) suppression of intensive anabolic processes-mainly photosynthesis and light-dependent reactions, (2) transition to effective catabolism and mobilization of carbohydrate reserve to meet the high energy demand of cells during microspore reprograming and (3) effective defense against stress-inducing treatment, i.e. protection of proper folding during protein biosynthesis and effective degradation of dysfunctional or damaged proteins. Additionally, 5.0 mu M AC enhanced the expression of all genes previously identified as being associated with embryogenic potential of microspores (TaTPD1-like, SERK and GSTF2). Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2022 Electronic address http://doi.org/10.1038/s41598-021-01671-y
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