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Neurohistology and Imaging Techniques
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SYSNO ASEP 0552493 Document Type M - Monograph Chapter R&D Document Type Monograph Chapter Title Stereomicroscopy in Neuroanatomy Author(s) Wilson, E. E. (US)
Chambers, W. (US)
Pelc, Radek (FGU-C) RID
Nothnagle, P. (US)
Davidson, M. W. (US)Source Title Neurohistology and Imaging Techniques. - New York : Springer, 2020 / Pelc R. ; Walz W. ; Doucette J. R. - ISSN 0893-2336 - ISBN 978-1-0716-0426-7 Pages s. 245-274 Number of pages 30 s. Number of pages 472 Publication form Print - P Language eng - English Country US - United States Keywords barrel distortion ; common main objective ; convergence ; diascopic illumination OECD category Biophysics R&D Projects LM2015062 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support FGU-C - RVO:67985823 EID SCOPUS 85089627582 DOI 10.1007/978-1-0716-0428-1_9 Annotation The main benefits of the stereomicroscope are that it is designed in a modular motif, allowing for a wide range of accessories such as stands, eyepieces, objectives, and illuminating bases for a wide variety of contrast enhancement techniques. Often utilized to study the surfaces of specimens, the stereomicroscope frequently uses incident (reflected) illumination, permitting the observation of specimens that would normally be too thick or opaque. Translucent and transparent objects can be successfully imaged with a number of transmitted illumination methods depending on the observer’s needs, and fluorescence stereomicroscopes are being increasingly used for three-dimensional observation. An excellent working distance, ranging from 3 to 5 cm to even 20 cm in certain models, and wide field of view that these models feature, are critical factors in the observation of a far-ranging variety of biological specimens. Examples of nervous and other tissues are presented in this review, for example, retina in living fish, paraneurons in shrimp, innervation of murine heart, and YFP-expressing regenerating nerves in the cornea of transgenic mice. Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2022 Electronic address https://doi.org/10.1007/978-1-0716-0428-1_9
Number of the records: 1